ISO INTERNATIONAL STANDARD 16000-18 First edition 2011-07-01 Indoor air - Part 18: Detection and enumeration of moulds Sampling by impaction Air intérieur Partie 18: Detection et denombrement des moisissures - Echantillonnage par impaction Reference number ISO 16000-18:2011(E) @ ISO 2011 py IHS under lic ted without license from IHS Not for Resale ISO 16000-18:2011(E) COPYRIGHTPROTECTEDDOCUMENT @ ISO2011 All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized in any form or by any means, Iso's member body in the country of the requester. ISO copyright office Case postale 56. CH-1211 Geneva 20 Tel. + 41 22 749 01 11 Fax + 41 22 749 09 47 E-mail copyright@iso.org Web www.iso.org Published in Switzerland @ ISO 2011 - All rights reserved Tby IHS under itted without license from IHS Not for Resale ISO 16000-18:2011(E) Contents Page Foreword Introductior 1 Scope 2 Normative references 3 Terms and definitions 4 Principle.. 5 Apparatus and materials... 5.1 Sampling device ... 5.2 Equipment for preparing the agar plates. 5.3 Equipment for sampling . 6 Culture media and reagents 6.1 General 6.2 Dichloran 18 % glycerol agar (DG18) 6.3 Malt-extract agar.... 6.4 Potatodextroseagar 7 Measurementprocedure.... 7.1 Preparation for sampling .. 7.2 Sampling...... 7.3 Sampling period and sampling volume 7.4 Transport and storage ... 8 Sampling efficiency and method limitations ...... 8 9 Calibration of flow rate, function control and maintenance of the sampling system ... .8 9.1 Calibration of flow rate.. 9.2 Function control and maintenance of the sampling system ... 10 Quality assurance. 8 11 Sampling protocol. 9 12 Performance characteristics. Annex A (informative) Technical description of a suitable one stage sieve impactor.. 10 Annex B (informative) Sampling protocol... 11 Annex C (informative) Sample exchange for method validation. 2 Bibliography.... .20 Copyrght International Organization forStandardizalon ghts reserved ii nse from IHS Not for Resale