论文标题
具有连续异质性的大分子的冷冻EM单粒子重建方法
Methods for Cryo-EM Single Particle Reconstruction of Macromolecules having Continuous Heterogeneity
论文作者
论文摘要
大分子在执行功能的过程中改变其形状（构象）。迅速冻干的冷冻电子显微镜成像，大分子（单个颗粒）的单个副本是一种强大而通用的方法，可理解大分子的运动和能量景观。广泛使用的计算方法已经允许从异质单粒子样品中恢复一些不同的构象，但是对复杂形式的异质性的处理，例如可能的短暂状态和柔性区域的连续体仍然很大程度上仍然是一个开放的问题。近年来，已经有了新的方法来处理更普遍的持续异质性问题。本文调查了该领域的当前最新技术状况。
Macromolecules change their shape (conformation) in the process of carrying out their functions. The imaging by cryo-electron microscopy of rapidly-frozen, individual copies of macromolecules (single particles) is a powerful and general approach to understanding the motions and energy landscapes of macromolecules. Widely-used computational methods already allow the recovery of a few distinct conformations from heterogeneous single-particle samples, but the treatment of complex forms of heterogeneity such as the continuum of possible transitory states and flexible regions remains largely an open problem. In recent years there has been a surge of new approaches for treating the more general problem of continuous heterogeneity. This paper surveys the current state of the art in this area.
