论文标题
碳纳米管底物增强了基质辅助激光解吸质谱质谱的SARS-COV-2尖峰蛋白离子的产量
Carbon nanotube substrates enhance SARS-CoV-2 spike protein ion yields in matrix assisted laser desorption-ionization mass spectrometry
论文作者
论文摘要
纳米结构的表面增强了基质辅助激光脱附质谱法（MALDI-MS）中的离子产量。 SPIKE蛋白复合物S1是SARS-COV-2的指纹特征，质量为75 kDa。在这里，我们表明，当将基质 - 分析液溶液放置在用未涂层的底物的产量相比，矩阵溶液的底物上，将基质 - 分析液放置在覆盖有密集的多壁碳纳米管森林的底物上时，SARS-COV-2尖峰蛋白离子的MALDI-MS产量增强了50倍。纳米结构的底物可以支持质谱技术的发展，以进行敏感的病原体检测和环境监测。
Nanostructured surfaces enhance ion yields in matrix assisted laser desorption-ionization mass spectrometry (MALDI-MS). The spike protein complex, S1, is one fingerprint signature of Sars-CoV-2 with a mass of 75 kDa. Here, we show that MALDI-MS yields of Sars-CoV-2 spike protein ions in the 100 kDa range are enhanced 50-fold when the matrix-analyte solution is placed on substrates that are coated with a dense forest of multi-walled carbon nanotubes, compared to yields from uncoated substrates. Nanostructured substrates can support the development of mass spectrometry techniques for sensitive pathogen detection and environmental monitoring.
