论文标题
使用1.8 $ $ m $ m飞秒激光系统的体内三光子和四光子荧光显微镜
In vivo three- and four-photon fluorescence microscopy using a 1.8 $μ$m femtosecond fiber laser system
论文作者
论文摘要
多光子显微镜使我们能够在体内图像细胞动力学。然而，用市售激光器进行成像的激发波长在650-1040 nm之间受到限制。在这里，我们开发了一个飞秒光纤激光系统，该系统生产$ \ sim $ 150 fs脉冲以1.8 $μ$ m。我们的系统始于掺杂的二氧化硅纤维激光器，其波长使用拉曼移位纤维将其波长转换为1.8 $μ$ m。 1.8 $μ$ M的脉冲用两阶段的TM：ZBLAN纤维放大器放大。最终的脉冲能量为$ \ sim $ 1 $μ$ J，足以体内成像。
Multiphoton microscopy has enabled us to image cellular dynamics in vivo. However, the excitation wavelength for imaging with commercially available lasers is mostly limited between 650-1040 nm. Here we develop a femtosecond fiber laser system that produces $\sim$150 fs pulses at 1.8 $μ$m. Our system starts from an erbium-doped silica fiber laser, and its wavelength is converted to 1.8 $μ$m using a Raman shift fiber. The 1.8 $μ$m pulses are amplified with a two-stage Tm:ZBLAN fiber amplifier. The final pulse energy is $\sim$1 $μ$J, sufficient for in vivo imaging.
