论文标题
半自动细胞跟踪过程，旨在完成秀丽隐杆线虫开发的4D地图集
A Semi-automatic Cell Tracking Process Towards Completing the 4D Atlas of C. elegans Development
论文作者
论文摘要
线虫秀丽隐杆线虫（秀丽隐杆线虫）被用作模型生物体，以更好地了解发育生物学和神经生物学。秀丽隐杆线虫具有不变的细胞谱系，已使用荧光显微镜图像进行了分类和观察。然而，一旦零星的肌肉抽搐开始，已经建立的跟踪晚期发育中细胞的方法就无法概括。我们以方法为基础，尽管随机抽搐，但使用皮肤细胞作为基金标记来进行细胞跟踪。特别是，我们提出了一个细胞核分割和跟踪程序，该过程被整合到3D渲染GUI中，以提高在晚期发育中跟踪细胞的效率。在三个测试胚胎上描述上述肌肉细胞核的图像上的结果表明，基准标记与经典的跟踪范式结合使用，克服了零星的抽搐。
The nematode Caenorhabditis elegans (C. elegans) is used as a model organism to better understand developmental biology and neurobiology. C. elegans features an invariant cell lineage, which has been catalogued and observed using fluorescence microscopy images. However, established methods to track cells in late-stage development fail to generalize once sporadic muscular twitching has begun. We build upon methodology which uses skin cells as fiducial markers to carry out cell tracking despite random twitching. In particular, we present a cell nucleus segmentation and tracking procedure which was integrated into a 3D rendering GUI to improve efficiency in tracking cells across late-stage development. Results on images depicting aforementioned muscle cell nuclei across three test embryos suggest the fiducial markers in conjunction with a classic tracking paradigm overcome sporadic twitching.
