学术文献库

Neurons are the basic cells of the nervous system. During their growth neurons extend two types of processes: axons and dendrites, which navigate to other neurons and form complex neuronal networks that transmit electrical signals throughout the body. The basic process underlying the network formation is axonal growth, a process involving the extension of axons from the cell body towards target neurons. During growth axons sense their environment, process information, and respond to external stimuli by adapting their growth dynamics. Environmental stimuli include intercellular interactions, biochemical cues, and the mechanical and geometrical features of the growth substrate. Axonal dynamics is controlled both by deterministic components (tendency to grow in certain preferred directions imparted by surface properties), and a random deviation from these growth directions due to stochastic processes. Despite recent impressive advances in our understanding of how neurons grow and form functional connections, a fully quantitative description of axonal dynamics is still missing. In this paper, we present a review of our work on stochastic models. We show that Langevin and Fokker-Planck stochastic differential equations provide a powerful theoretical framework for describing how collective axonal dynamics and the formation of neuronal network emerge from biophysics of single neurons and their interactions. We combine this theoretical analysis with experimental data to extract key parameters of axonal growth: diffusion coefficients, speed and angular distributions, mean square displacements, and mechanical parameters describing the cell-substrate coupling. These results have important implications for our understanding of the fundamental mechanisms involved in the formation of functional neuronal networks, as well as for bioengineering novel scaffolds to promote nerve regeneration.