论文标题
通过DNA弯曲蛋白的纠缠的流体化
Fluidification of entanglements by a DNA bending protein
论文作者
论文摘要
尽管纳米级和单分子的见解对核苷相关蛋白（NAP）如何与DNA相互作用，但到目前为止，它们在调节体内纠缠基因组的中尺度粘弹性中的作用已被忽略了。通过结合微流变学和分子动力学模拟，我们发现称为Integration宿主因子（IHF）的重要NAP降低了纠缠$λ$ DNA的粘度，在生理浓度和稳定的时分时20倍。我们认为IHF可以充当“基因组流体”，从而降低了核苷$ \ sim $ 200倍的有效粘度。我们的结果表明，IHF在调节体内的DNA动力学和重组中的先前未批准的关键作用
In spite of the nanoscale and single-molecule insights into how nucleoid associated proteins (NAPs) interact with DNA, their role in modulating the mesoscale viscoelasticity of the entangled genome in vivo has been overlooked so far. By combining microrheology and molecular dynamics simulation we find that the important NAP called Integration Host Factor (IHF) lowers the viscosity of entangled $λ$DNA 20-fold at physiological concentrations and stoichiometries. We argue that IHF may act as a "genomic fluidiser", reducing the effective viscosity of the nucleoid $\sim$200-fold. Our results suggest a previously unappreciated key role of IHF in regulating DNA dynamics and re-organisation in vivo
