论文标题
由曲率诱导蛋白诱导的膜形状变形，由手性新月结合和内在无序结构域组成
Membrane shape deformation induced by curvature-inducing proteins consisting of chiral crescent binding and intrinsically disordered domains
论文作者
论文摘要
含有垃圾箱/两亲蛋白/RVS结构域的曲率诱导蛋白通常具有内在无序的结构域。最近的实验表明，这些无序链增强了曲率感应和产生。在这里，我们报告了使用无网状膜模拟通过无序链对蛋白质膜相互作用的修饰。将蛋白质和结合的膜一起建模为手性新月形蛋白棒，并具有两个排除的体积链。随着链长的增加，它们之间的排斥会减小蛋白质的簇大小。它诱导主轴形囊泡，并在圆盘形囊泡中的弧形和圆形蛋白质组件之间进行过渡。对于平坦的膜，由于蛋白质组件之间的排斥，中间链长会诱导许多小管，而较长的链条促进小管的垂直伸长。此外，具有零杆曲率和足够长链的蛋白质棒可以稳定球形芽。对于具有负杆曲率的蛋白质，中间链长的长度可诱导带有分支蛋白质组件的坚固膜，而较长的链条诱导具有周期性凹环结构的小管形成。
Curvature-inducing proteins containing a Bin/Amphiphysin/Rvs domain often have intrinsically disordered domains. Recent experiments have shown that these disordered chains enhance curvature sensing and generation. Here, we report on the modification of protein-membrane interactions by disordered chains using meshless membrane simulations. The protein and bound membrane are modeled together as a chiral crescent protein rod with two excluded-volume chains. As the chain length increases, the repulsion between them reduces the cluster size of the proteins. It induces spindle-shaped vesicles and a transition between arc-shaped and circular protein assemblies in a disk-shaped vesicle. For flat membranes, an intermediate chain length induces many tubules owing to the repulsion between the protein assemblies, whereas longer chains promote perpendicular elongation of tubules. Moreover, protein rods with zero rod curvature and sufficiently long chains stabilize the spherical buds. For proteins with a negative rod curvature, an intermediate chain length induces a rugged membrane with branched protein assemblies, whereas longer chains induce the formation of tubules with periodic concave-ring structures.
