论文标题
具有数字全息显微镜的活体微藻培养物的非侵入性脂质定量
Non-invasive lipid quantification of living microalgal cultures with digital holographic microscopy
论文作者
论文摘要
一些微藻在脂质液滴中存放大量中性脂质。由于这些脂质可用于以可持续的方式生产生物柴油，因此正在对快速无损方法进行研究，以量化和监测微藻培养物中脂质的量。在本文中，我们使用数字全息显微镜开发了一种快速定量方法，可以评估硅藻三乳酸乳酸化含量三角肌活细胞内的脂质含量的演变。该方法基于数字全息显微镜的重新关注能力，使用了记录全息图序列的特定处理。每个脂质液滴体积都在细胞内部的代表性样品内评估。由于相关定量相对比荧光成像，我们已经验证了该方法，并将其推断到较大的校准球形折射颗粒中，以证明该方法的灵活性。
Some microalgae store large amounts of neutral lipids inside lipid droplets. Since these lipids can be used to produce biodiesel in a sustainable way, research is developing on fast non-destructive methods to quantify and monitor the amount of lipids in microalgal cultures. In this paper, we have developed with digital holographic microscopy a fast quantitative method to assess the evolution of the lipid content inside the diatom Phaeodactylum tricornutum living cells. The method uses a specific processing of recorded hologram sequences based on the refocusing capability of digital holographic microscopy. Each lipid droplet volume is evaluated inside the cells on representative samples of the culture. We have validated the method thanks to correlative quantitative phase contrast-fluorescence imaging and extrapolated it to larger calibrated spherical refractive particles, to demonstrate the flexibility of the method.
