论文标题
纳米原子量子传感器揭示了与海马神经元发射相关的温度变化
Nanodiamond quantum sensors reveal temperature variation associated to hippocampal neurons firing
论文作者
论文摘要
温度是调节细胞内过程的最相关参数之一。测量局部亚细胞温度梯度是对细胞功能的更深入了解，例如作用电位的起源和细胞代谢。在这里，我们检测到与神经元发射的增强和耗竭相关的第一次温度变化（1°C），利用基于纳米座中光学检测到的磁共振的纳米级温度计。我们的结果提供了一种在生理和病理条件下评估神经元峰值活性的工具，并与该技术的高灵敏度相结合（从透视敏感到<0.1°C变化），为对局部温度梯度产生的系统研究铺平了道路。此外，他们促使进一步的研究详细解释了生理机制起源于这种作用。
Temperature is one of the most relevant parameters for the regulation of intracellular processes. Measuring localized subcellular temperature gradients is fundamental for a deeper understanding of cell function, such as the genesis of action potentials, and cell metabolism. Here, we detect for the first time temperature variations (1°C) associated with potentiation and depletion of neuronal firing, exploiting a nanoscale thermometer based on optically detected magnetic resonance in nanodiamonds. Our results provide a tool for assessing neuronal spiking activity under physiological and pathological conditions and, conjugated with the high sensitivity of this technique (in perspective sensitive to < 0.1°C variations), pave the way to a systematic study of the generation of localized temperature gradients. Furthermore, they prompt further studies explaining in detail the physiological mechanism originating this effect.
