论文标题
通过体积动态光学相干断层扫描的非酒精性 - 脂肪酶（NAFLD）肝脏（NAFLD）的无标签代谢成像
Label-free metabolic imaging of non-alcoholic-fatty-liver-disease (NAFLD) liver by volumetric dynamic optical coherence tomography
论文作者
论文摘要
通过动态光学相干断层扫描（OCT）证明了非酒精性脂肪肝病（NAFLD）小鼠肝脏（NAFLD）的无标记代谢成像。 NAFLD小鼠是一种蛋氨酸胆碱缺陷型（MCD） - diet模型，除了正常的小鼠外，还涉及两只喂MCD饮食1和2周的小鼠。动态OCT基于重复的栅格扫描和对数强度方差（LIV）分析，该分析能够使用标准速度（50,000 a-line/s）OCT系统实现体积代谢成像。与脂质液滴积累和炎症相关的代谢结构域在三维情况下清楚地看到。特别是，正常肝脏表现出血管周围肝运动区的高代谢血管结构。 1周的MCD-DIET肝脏显示由脂质液滴形成的环形高代谢结构。 2周的MCD-DIET肝脏表现出与炎症相关的碎片血管样结构。这些结果表明，体积LIV成像可用于可视化和评估NAFLD异常。
Label-free metabolic imaging of non-alcoholic fatty liver disease (NAFLD) mouse liver is demonstrated ex vivo by dynamic optical coherence tomography (OCT). The NAFLD mouse is a methionine choline-deficient (MCD)-diet model, and two mice fed MCD diet for 1 and 2 weeks are involved in addition to a normal-diet mouse. The dynamic OCT is based on repeating raster scan and logarithmic intensity variance (LIV) analysis which enables volumetric metabolic imaging with a standard-speed (50,000 A-lines/s) OCT system. Metabolic domains associated with lipid droplet accumulation and inflammation are clearly visualized three-dimensionally. Particularly, the normal-diet liver exhibits highly metabolic vessel-like structures of peri-vascular hepatic zones. The 1-week MCD-diet liver shows ring-shaped highly metabolic structures formed with lipid droplets. The 2-week MCD-diet liver exhibits fragmented vessel-like structures associated with inflammation. These results imply that volumetric LIV imaging is useful for visualizing and assessing NAFLD abnormalities.
