论文标题
直接测量细菌悬浮液中的Aerotactic响应
Direct measurement of the aerotactic response in a bacterial suspension
论文作者
论文摘要
空气障碍是通向氧气导航的能力。一个关键的问题是局部氧浓度c的依赖性速度c。在这里，我们使用胶束中包裹的钌构成的弦齿来表征Burkholderia污染物的Aerotactic响应，这是一种环境中易月细菌。在我们的实验中，密封的玻璃毛细管中的细菌呼吸可在一端渗透到氧气中，从而产生氧梯度，从而产生向氧气源传播的细菌带。我们在种群尺度上，从细菌带的漂移速度和细菌尺度上计算自动响应χ（C），从运行时间的角度调节。两种方法都与幂律χ\ propto c^{ - 2}一致，与基于细菌膜受体的生物化学的现有模型吻合。
Aerotaxis is the ability of motile cells to navigate toward oxygen. A key question is the dependence of the aerotactic velocity with the local oxygen concentration c. Here we combine simultaneous bacteria tracking and local oxygen concentration measurements using Ruthenium encapsulated in micelles to characterize the aerotactic response of Burkholderia contaminans, a motile bacterium ubiquitous in the environment. In our experiments, an oxygen gradient is produced by the bacterial respiration in a sealed glass capillary permeable to oxygen at one end, producing a bacterial band traveling toward the oxygen source. We compute the aerotactic response χ(c) both at the population scale, from the drift velocity in the bacterial band, and at the bacterial scale, from the angular modulation of the run times. Both methods are consistent with a power-law χ\propto c^{-2}, in good agreement with existing models based on the biochemistry of bacterial membrane receptors.
