论文标题
动态光学对比度成像，用于使用头颈癌作为模型的肿瘤切除缘实时描述
Dynamic optical contrast imaging for real-time delineation of tumor resection margins using head and neck cancer as a model
论文作者
论文摘要
鉴于侵略性手术的毁灭性副作用以及与重要结构的解剖结构，肿瘤的完全手术切除（HNSCC）仍然具有挑战性。为了应对临床挑战，我们引入了一个宽阔的无标签成像工具，可以帮助外科医生实时描绘肿瘤边缘。我们假设自动荧光寿命是组织健康水平的自然指标，并且比率测量发射率态与激发荧光团激发的发射峰状态将实现组织的快速寿命映射。在这里，我们描述了成像仪的原理，仪器，表征和术中成像的术中成像，来自13例接受头颈癌切除的患者的切除组织。 20 x 20 mm2成像的工作距离为50 mm，框架为2秒/帧，表征表明空间分辨率达到70μm，最少可区分的荧光寿命差为0.14 ns。组织成像和苏木精 - 欧洲蛋白染色幻灯片比较揭示了其以亚毫米准确性和91.86％的敏感性和84.38％的特异性来描述癌细胞边界的能力。
Complete surgical resection of the tumor for Head and neck squamous cell carcinoma (HNSCC) remains challenging, given the devastating side effects of aggressive surgery and the anatomic proximity to vital structures. To address the clinical challenges, we introduce a wide-field, label-free imaging tool that can assist surgeons delineate tumor margins real-time. We assume that autofluorescence lifetime is a natural indicator of the health level of tissues, and ratio-metric measurement of the emission-decay state to the emission-peak state of excited fluorophores will enable rapid lifetime mapping of tissues. Here, we describe the principle, instrumentation, characterization of the imager and the intraoperative imaging of resected tissues from 13 patients undergoing head and neck cancer resection. 20 x 20 mm2 imaging takes 2 second/frame with a working distance of 50 mm, and characterization shows that the spatial resolution reached 70 μm and the least distinguishable fluorescence lifetime difference is 0.14 ns. Tissue imaging and Hematoxylin-Eosin stain slides comparison reveals its capability of delineating cancerous boundaries with submillimeter accuracy and a sensitivity of 91.86% and specificity of 84.38%.
