论文标题
微量滴定板中的对流增强动力学，用于改进表面测定定量和多路复用能力
Advection-enhanced kinetics in microtiter plates for improved surface assay quantitation and multiplexing capabilities
论文作者
论文摘要
诸如ELISA之类的表面测定在诊所和研究中普遍存在，并且在微量滴定板（MTP）中主要是标准化的。 MTP提供了许多优势，但由于固有的质量运输限制，通常不利于表面测定效率。微观流可以克服这些，并在很大程度上改善了测定动力学。但是，具有现有实验室软件和协议的微流体的破坏性范围缩小了其变革性的潜力。我们提出了Wellprobe，这是一种与MTPS兼容的新型微流体概念。有了它，我们表明免疫测定在低浓度下变得更敏感（在12倍的时间内提高了9倍的信号改善），具有3-4个不同动力学条件的信息丰富，可用于估计动力学参数，最小化洗涤步骤和非特异性的结合，并确定结果。我们进一步多重单孔测定，将井探针动力学区域与量身定制的微阵列相结合。最后，我们在免疫球蛋白亚类评估的背景下演示了我们的系统，该评估越来越被视为临床相关。
Surface assays such as ELISA are pervasive in clinics and research and predominantly standardized in microtiter plates (MTP). MTPs provide many advantages but are often detrimental to surface assay efficiency due to inherent mass transport limitations. Microscale flows can overcome these and largely improve assay kinetics. However, the disruptive nature of microfluidics with existing labware and protocols has narrowed its transformative potential. We present WellProbe, a novel microfluidic concept compatible with MTPs. With it, we show that immunoassays become more sensitive at low concentrations (up to 9-fold signal improvement in 12-fold less time), richer in information with 3-4 different kinetic conditions, and can be used to estimate kinetic parameters, minimize washing steps and non-specific binding, and identify compromised results. We further multiplex single-well assays combining WellProbes kinetic regions with tailored microarrays. Finally, we demonstrate our system in a context of immunoglobulin subclass evaluation, increasingly regarded as clinically relevant.
