论文标题
为什么可以通过大型捕获生物电子界面在几分钟内检测到分散的单分子
Why a diffusing single-molecule can be detected in few minutes by a large capturing bioelectronic interface
论文作者
论文摘要
在Femtolor溶液中，在纳米界面处的单分子检测可能需要数周的时间，因为要检测到的扩散分子与转导纳米脱位之间的相遇速率忽略很小。另一方面，一些实验证明，基于现场效应 - 透射器（FET）的宏观标记传感器，可引人入胜的微米或毫米检测接口能够在几分钟内大量的单分子分析单分子。目前的工作证明了为什么在100 ul体积中至少散布的至少一个分子具有很高的可能性，可以击中大型捕获和检测电子界面。为此，在这里提供了用电解质门控的FET测量的传感数据，其门与1012捕获抗免疫球蛋白G的栅极和基于Brownian扩散的建模一起提供。 EG-FET测定溶液的浓度降低至几十亿美元，体积范围从25 ul到1 ml，在该ZM中，功能化的门在30 s到20分钟的范围内孵育。
Single-molecule detection at a nanometric interface in a femtomolar solution, can take weeks as the encounter rate between the diffusing molecule to be detected and the transducing nano-device is negligibly small. On the other hand, several experiments prove that macroscopic label-free sensors based on field-effect-transistors (FET), engaging micrometric or millimetric detecting interfaces are capable to assay a single-molecule in a large volume within few minutes. The present work demonstrates why at least a single molecule out of a few diffusing in a 100 ul volume has a very high probability to hit a large capturing and detecting electronic interface. To this end, sensing data, measured with an electrolyte-gated FET whose gate is functionalized with 1012 capturing anti-immunoglobulin G, are here provided along with a Brownian diffusion-based modelling. The EG-FET assays solutions down to some tens of zM in concentrations with volumes ranging from 25 ul to 1 ml in which the functionalized gates are incubated for times ranging from 30 s to 20 min.
