论文标题
亚毫米柔性纤维探针，用于背景和荧光无拉曼光谱
Sub millimetre flexible fibre probe for background and fluorescence free Raman spectroscopy
论文作者
论文摘要
使用偏移的拉曼差异光谱技术和具有负曲率激发芯和高数值孔径收集核的同轴环的光纤，我们开发了一种便携式，背景和荧光无内窥镜的拉曼探针。该探针由单个纤维组成，直径小于0.25毫米，该纤维在亚毫米管中包装，使其与标准支气管镜兼容。纤维中的拉曼激发灯在空气中被引导，因此与二氧化硅相互作用几乎没有相互作用，从而使兴奋光的几乎无背景传播。此外，我们使用了移位的拉曼差异光谱技术和可调的785 nm激光器来将荧光和拉曼光谱与高度荧光样品分开，这表明了探针对生物医学应用的适用性。使用此探针，我们还获得了无荧光的无肺组织数据。
Using the shifted-excitation Raman difference spectroscopy technique and an optical fibre featuring a negative curvature excitation core and a coaxial ring of high numerical aperture collection cores, we have developed a portable, background and fluorescence free, endoscopic Raman probe. The probe consists of a single fibre with a diameter of less than 0.25 mm packaged in a sub-millimetre tubing, making it compatible with standard bronchoscopes. The Raman excitation light in the fibre is guided in air and therefore interacts little with silica, enabling an almost background free transmission of the excitation light. In addition, we used the shifted-excitation Raman difference spectroscopy technique and a tunable 785 nm laser to separate the fluorescence and the Raman spectrum from highly fluorescent samples, demonstrating the suitability of the probe for biomedical applications. Using this probe we also acquired fluorescence free human lung tissue data.
