论文标题
细菌是幽闭恐惧症吗？微生物培养的微米空间限制问题
Are bacteria claustrophobic? The problem of micrometric spatial confinement for the culture of micro-organisms
论文作者
论文摘要
在过去的十年中，已经报道了许多研究，尤其是在基于微流体的应用和实验室芯片设备的开发之后，已经报道了限制在微观几何形状中的培养细胞。这些研究通常检查大肠杆菌的生长。在本文中，我们表明大肠杆菌可能是一个很差的模型，并且空间限制可以严重防止许多微生物的生长。通过研究不同的细菌和限制几何形状，我们确定某些细菌观察到的生长抑制作用是由于快速的二氧化物耗竭而导致的，这是空间限制固有的，而不是营养耗尽的。本文揭示了细胞培养中限制问题的物理起源，强调了氧气耗竭的重要性，并通过证明气泡附近的几何形状中的细胞生长来增强细胞的生长，为细菌的有效培养铺平了道路。
Culturing cells confined in microscale geometries has been reported in many studies this last decade, in particular following the development of microfluidic-based applications and lab-on-a-chip devices. Such studies usually examine growth of Escherichia coli. In this article, we show that E. coli may be a poor model and that spatial confinement can severely prevent the growth of many micro-organisms. By studying different bacteria and confinement geometries, we determine that the growth inhibition observed for some bacteria results from fast dioxygen depletion, inherent to spatial confinement, and not to any depletion of nutriments. This article unravels the physical origin of confinement problems in cell culture, highlighting the importance of oxygen depletion, and paves the way for the effective culture of bacteria in confined geometries by demonstrating enhanced cell growth in confined geometries in the proximity of air bubbles.
