论文标题
核苷的紫外线耐药性 - 一种实验方法
UV resistance of nucleosides -- an experimental approach
论文作者
论文摘要
地球上生命的出现是一个高度讨论但仍未解决的问题。当前的研究强调了环境在接近地球表面的重要性，因为它们可以解决长期存在的问题，例如核苷酸的聚合和核苷的磷酸化。但是，近地表设置，例如池塘或冰盾，容易受到紫外线照射。我们通过使用拉曼显微镜研究了尿嘧啶，尿苷，腺苷，胞苷和鸟苷的光敏性。样品被带有150〜MW/cm \ textsuperscript {2}的UV源辐照10分钟。尿嘧啶和尿疗表现出最高的光敏性，而腺苷，胞苷和鸟嘌呤保持稳定。光谱特征的变化和从头量的量子计算表明，在紫外线照射期间，乌拉西尔的反式循环二聚体形成。
The emergence of life on Earth is a highly discussed but still unsolved question. Current research underlines the importance of environments within close proximity to the Earth's surface as they can solve long-standing problems such as polymerization of nucleotides and phosphorylation of nucleosides. However, near-surface settings, for example, ponds or ice shields, are prone to UV irradiation. We investigated the photosensitivity of uracil, uridine, adenosine, cytidine, and guanosine by using Raman microscopy. The samples were irradiated by a UV source with 150~mW/cm\textsuperscript{2} for 10 min. Uracil and uridine showed the highest photosensitivity, while adenosine, cytidine, and guanosine remained stable. The change of spectral features and ab initio quantum calculations indicate the formation of uracil's trans-syn cyclobutane dimer during UV irradiation.
