论文标题
桥接引起的微体分离：光漂白实验，染色质结构域和对主动反应的需求
Bridging-induced microphase separation: photobleaching experiments, chromatin domains, and the need for active reactions
论文作者
论文摘要
我们回顾了“桥接引起的吸引力”的机制和后果，这是一种通用的生物物理原理，为3-D中的一些现有染色体组织的模型构成了一些现有模型。这种吸引在聚合物物理启发的计算机模拟中揭示了这种吸引力，是一种在多价染色质结合蛋白中产生的通用聚类趋势，它为核体和转录工厂的生物发生提供了解释。包括涉及这些多价蛋白的翻译后修饰反应可以解释随后簇的快速动态，正如通过显微镜和光漂白实验所观察到的。在模拟中发现的簇还产生了染色质结构域，这些结构域与HIC实验中对A/B隔室的观察非常吻合。
We review the mechanism and consequences of the "bridging-induced attraction", a generic biophysical principle which underpins some existing models for chromosome organisation in 3-D. This attraction, which was revealed in polymer physics-inspired computer simulations, is a generic clustering tendency arising in multivalent chromatin-binding proteins, and it provides an explanation for the biogenesis of nuclear bodies and transcription factories via microphase separation. Including post-translational modification reactions involving these multivalent proteins can account for the fast dynamics of the ensuing clusters, as is observed via microscopy and photobleaching experiments. The clusters found in simulations also give rise to chromatin domains which conform well with the observation of A/B compartments in HiC experiments.
