论文标题
细胞壁对降解的抗性是从枯草芽孢杆菌的天然分离株中分离高质量RNA的关键参数
Resistance of the cell wall to degradation is a critical parameter for isolation of high quality RNA from natural isolates of Bacillus subtilis
论文作者
论文摘要
枯草芽孢杆菌的天然分离株以产生大量生物活性化合物的能力而闻名。不幸的是，它们对常规分子技术的顽固性限制了其转录本研究。在这项工作中，克服了归因于细胞壁的Diyculties，可以授权强大的RT-PCR。
Natural isolates of Bacillus subtilis are known for their ability to produce a large panel of bioactive compounds. Unfortunately, their recalcitrance to conventional molecular techniques limits their transcript studies. In this work, diYculties to isolate RNA attributed to the cell wall were overcome, Wnally authorising powerful RT-PCR's.
