论文标题
菠萝（Ananas comosus）顶端的冷冻保存
Cryopreservation of pineapple (Ananas comosus) apices
论文作者
论文摘要
试验了封装脱水和玻璃化技术，以冻结菠萝的体外植物。仅使用玻璃化实现了积极的结果。最佳条件包括在补充了0.3m蔗糖的培养基上的2-D先生条件，在培养基中加载25分钟，蔗糖 + 1m甘油 + 1m甘油和脱水，在0度C上用PVS2玻璃化溶液脱水7 h，在液态氮气中快速冷冻之前，在0度C中持续7 h。该方法分别与波多黎各，佩罗雷拉和光滑的辣椒的品种的顶点产生了65％，35％和25％的生存率。冷冻保存的顶点的恢复直接发生，而没有短暂的愈伤组织形成。
The encapsulation-dehydration and vitrification techniques were experimented for freezing apices of pineapple in vitro plantlets. Positive results were achieved using vitrification only. Optimal conditions included a 2-d preculture of apices on medium supplemented with 0.3M sucrose, loading treatment for 25 min in medium with 0.75M sucrose + 1M glycerol and dehydration with PVS2 vitrification solution at 0 degrees C for 7 h before rapid freezing in liquid nitrogen. This method resulted in 65, 35 and 25% survival with apices of varieties Puerto Rico, Perolera and Smooth Cayenne, respectively. Recovery of cryopreserved apices took place directly, without transitory callus formation.
