论文标题
使用光散射光谱法监测组织模型的折射率
Monitoring the refractive index of tissue models using light scattering spectroscopy
论文作者
论文摘要
在这项工作中，我们报告了拉曼微光谱镜用于分析一系列组织幻象的折射率的应用。使用带有可见激光源的定制设置和带有近红外激光的商业微光谱计，我们以各种浓度测量了明胶水凝胶的拉曼光谱。通过从测得的折射率数据和拉曼散射强度为水凝胶的不同振动模式构建校准曲线，我们确定了从拉曼光谱中的凝胶的折射率，其准确性与用ABBE倒流仪的冰期测量测量的精度大致相同。这项工作强调了通过Brillouin-Raman微光谱法对生物学相关样品进行机械化学分析的相关方法的重要性。
In this work, we report the application of Raman microspectroscopy for analysis of the refractive index of a range of tissue phantoms. Using both a custom-developed setup with visible laser source and a commercial micro-spectrometer with near infrared laser, we measured the Raman spectra of gelatin hydrogels at various concentrations. By building a calibration curve from measured refractometry data and Raman scattering intensity for different vibrational modes of the hydrogel, we determined the refractive indices of the gels from their Raman spectra, with approximately the same accuracy as that of refractometry measurements with an Abbe refractometer. This work highlights the importance of a correlative approach through Brillouin-Raman microspectroscopy for the mechano-chemical analysis of biologically relevant samples.
