论文标题
使用简化的冷冻过程，甘蔗胚胎愈伤组织的冷冻保存
Cryopreservation of sugarcane embryogenic callus using a simplified freezing process
论文作者
论文摘要
开发了一个简化的冷冻过程，并成功地应用于三种甘蔗商业杂种（Saccharumsp。cv。CP5243，C 91-301和C 1051-73）的胚胎call虫。为了获得最佳的存活，用含有10％DMSO和0.3至0.75m蔗糖的冷冻保护溶液预处理老茧。为了冷冻，将样品浸入放置在-40摄氏度的冰箱中的酒精浴中，从而允许冻结速率在0.4至0.6摄氏度之间。在浸入液氮之前，将样品保持在该温度2小时。冷冻保存的升藻的生存率最高的范围在20％至94％之间，具体取决于品种，并且可以通过重生的老茧获得完全开发的花序。将一种品种的胚胎发生在液氮中存储14个月，对其存活率和植物生产产生任何影响。
A simplified freezing process was developed and successfully applied to embryogenic calluses of three sugarcane commercial hybrids (Saccharum sp. cv. CP 5243, C 91-301 and C 1051-73). To obtain optimal survival, the calluses were pretreated with a cryoprotective solution containing 10% DMSO and 0.3 to 0.75M sucrose. For freezing, the samples were immersed in an alcohol bath placed in a -40 degrees C freezer, thus allowing a freezing rate comprised between 0.4 and 0.6 degrees C/min. Samples were held at that temperature for 2 h before immersion in liquid nitrogen. The highest survival rates of cryopreserved calluses ranged between 20 and 94% depending on the variety, and fully developed plantlets could be obtained from regenerating calluses. Embryogenic calluses of one variety were stored for 14 months in liquid nitrogen without any effect on their survival rate and plantlet production.
