论文标题
通过无柄滴法的相分离蛋白和聚合物液滴的表面张力仪法
Surface tensiometry of phase separated protein and polymer droplets by the sessile drop method
论文作者
论文摘要
分离的大分子在许多生物学和合成系统中起着重要作用。由于样品量有限，特别是对于相分离的蛋白质，这些系统的物理表征可能具有挑战性。在这里，我们证明了一种经典的方法，可以根据该应用有效地缩放液滴表面液滴的表面张力。液滴形状和表面张力之间的连接取决于液滴及其周围环境之间的密度差。可以通过测量液滴沉积速度在同一设置中用少量样品体积确定。 ESI中包含用于从液滴图像中提取毛细管长度的交互式MATLAB脚本。
Phase separated macromolecules play essential roles in many biological and synthetic systems. Physical characterization of these systems can be challenging because of limited sample volumes, particularly for phase-separated proteins. Here, we demonstrate that a classic method for measuring the surface tension of liquid droplets, based on the analysis of the shape of a sessile droplet, can be effectively scaled down for this application. The connection between droplet shape and surface tension relies on the density difference between the droplet and its surroundings. This can be determined with small sample volumes in the same setup by measuring the droplet sedimentation velocity. An interactive MATLAB script for extracting the capillary length from a droplet image is included in the ESI.
