论文标题
PepSirf：一种灵活而全面的工具，用于分析来自高度多形的DNA-Barcoded肽分析的数据
PepSIRF: a flexible and comprehensive tool for the analysis of data from highly-multiplexed DNA-barcoded peptide assays
论文作者
论文摘要
通过将肽与DNA标签耦合（即“条形码”），现在可以利用高通量测序（HTS）技术启用高度多重肽的测定法，这些测定具有多种潜在应用，包括抗体识别的表位表位。通常，HTS数据的处理已经得到很好的支持，但很少有软件工具开发出来，用于处理这些高度磁性肽分析中生成的数据。为了填补这一空白，我们提出了PepSirf（基于肽的血清学免疫反应框架），该框架是一个灵活而全面的软件包，专门针对来自高度多形肽基于肽的测定的HTS数据而设计。
By coupling peptides with DNA tags (i.e., 'barcodes'), it is now possible to harness high-throughput sequencing (HTS) technologies to enable highly multiplexed peptide-based assays, which have a variety of potential applications including broad characterization of the epitopes recognized by antibodies. While the processing of HTS data, in general, is already well supported, there are very few software tools that have been developed for working with data generated in these highly-multiplexed peptide assays. In order to fill this gap, we present PepSIRF (Peptide-based Serological Immune Response Framework), which is a flexible and comprehensive software package designed specifically for the analysis of HTS data from highly-multiplexed peptide-based assays.
