论文标题
合作和非相同分子电动机的有效行为
Effective behavior of cooperative and nonidentical molecular motors
论文作者
论文摘要
用于有效漂移,扩散率,运行时间和运行长度的分析公式是针对由附着在两个合作但不相同的分子电机(例如kinein-1和kinesin-2)组成的细胞内运输系统的,它们每个都可以连接并脱离微管。每个阶段的电动机和货物的动力学都由随机微分方程控制,开关速率取决于电动机和货物的空间配置。该系统以比货物的动力更快的限制进行分析,而货物的动态速度比附着的电动机更快。相对于空间动力学,附着和脱离率也被认为是缓慢的。通过将迭代的随机平均应用于该系统的应用,并使用更新 - 奖励理论将每个开关阶段的进展拼接在一起,我们获得了明确的分析表达式,以实现有效的漂移,扩散率和摄影系统的加工性。我们的方法尤其是在固定和分离事件期间发生的电动机位置的跳跃,因为货物跟踪变量由于平均快速尺度而进行了快速调整。渐近公式通常与基于实验参数的详细模型的直接随机模拟在辅助,阻碍或无负载下的各种配对的详细模型的直接仿真。
Analytical formulas for effective drift, diffusivity, run times, and run lengths are derived for an intracellular transport system consisting of a cargo attached to two cooperative but not identical molecular motors (for example, kinesin-1 and kinesin-2) which can each attach and detach from a microtubule. The dynamics of the motor and cargo in each phase are governed by stochastic differential equations, and the switching rates depend on the spatial configuration of the motor and cargo. This system is analyzed in a limit where the detached motors have faster dynamics than the cargo, which in turn has faster dynamics than the attached motors. The attachment and detachment rates are also taken to be slow relative to the spatial dynamics. Through an application of iterated stochastic averaging to this system, and the use of renewal-reward theory to stitch together the progress within each switching phase, we obtain explicit analytical expressions for the effective drift, diffusivity, and processivity of the motor-cargo system. Our approach accounts in particular for jumps in motor-cargo position that occur during attachment and detachment events, as the cargo tracking variable makes a rapid adjustment due to the averaged fast scales. The asymptotic formulas are in generally good agreement with direct stochastic simulations of the detailed model based on experimental parameters for various pairings of kinesin-1 and kinesin-2 under assisting, hindering, or no load.