论文标题
具有快速宽带傅立叶转换连贯拉曼模态的多模式激光扫描非线性光学显微镜
A multimodal laser-scanning nonlinear optical microscope with a rapid broadband Fourier-transform coherent Raman modality
论文作者
论文摘要
非线性光学显微镜允许快速高分辨率显微镜与样品内在特性产生的图像对比度。建立的模态（例如多光子激发荧光和第二/第三谐波生成）可以与其他非线性技术相结合，例如相干拉曼光谱法，通常可以化学成像样品的单个共振振动模式。在这里，我们利用单个超快激光源在显微镜上获得宽带相干拉曼光谱，并在同一仪器上使用其他非线性显微镜方法。我们证明，连贯的拉曼模态允许具有高光谱分辨率（<5 cm-1）的宽带测量（> 1000 cm-1），并具有快速的光谱采集率（3-12 kHz）。这使拉曼高光谱成像>公斤像素图像以每秒> 11帧的速度。
Nonlinear optical microscopy allows rapid high-resolution microscopy with image contrast generated from intrinsic properties of the sample. Established modalities such as multiphoton excited fluorescence and second/third-harmonic generation can be combined with other nonlinear techniques, such as coherent Raman spectroscopy which typically allow chemical imaging of a single resonant vibrational mode of a sample. Here, we utilize a single ultrafast laser source to obtain broadband coherent Raman spectra on a microscope, together with other nonlinear microscopy approaches on the same instrument. We demonstrate that the coherent Raman modality allows broadband measurement (>1000 cm-1), with high spectral resolution (<5 cm-1), with a rapid spectral acquisition rate (3-12 kHz). This enables Raman hyperspectral imaging of > kilo-pixel images at >11 frames per second.
