论文标题
高尔基体中的聚糖处理 - 最佳信息编码和脑海数和酶特异性的约束
Glycan processing in the Golgi -- optimal information coding and constraints on cisternal number and enzyme specificity
论文作者
论文摘要
许多在高尔基水库中进行顺序酶促修饰的蛋白在质膜上显示为细胞身份标记。改性蛋白（称为Glycans）代表分子代码。该聚糖代码的忠诚度是通过如何准确地测量聚糖合成机械来实现特定细胞类型和利基市场所需的目标聚糖分布的程度。在本文中，我们定量分析了水库数量与酶的数量和特异性之间的权衡，以综合某个规定的目标聚糖分布的某个复杂性。我们发现，要合成复杂分布，例如在真实细胞中观察到的分布，需要在高尔基体中具有多个蓄水池和精确的酶分配。此外，对于固定数量的酶和水库，具有最佳的酶特异性水平，可以以高忠诚度实现目标分布。我们的结果表明，目标聚糖分布的复杂性如何将功能约束放在高尔基体蓄水池数和酶特异性上。
Many proteins that undergo sequential enzymatic modification in the Golgi cisternae are displayed at the plasma membrane as cell identity markers. The modified proteins, called glycans, represent a molecular code. The fidelity of this glycan code is measured by how accurately the glycan synthesis machinery realises the desired target glycan distribution for a particular cell type and niche. In this paper, we quantitatively analyse the tradeoffs between the number of cisternae and the number and specificity of enzymes, in order to synthesize a prescribed target glycan distribution of a certain complexity. We find that to synthesize complex distributions, such as those observed in real cells, one needs to have multiple cisternae and precise enzyme partitioning in the Golgi. Additionally, for fixed number of enzymes and cisternae, there is an optimal level of specificity of enzymes that achieves the target distribution with high fidelity. Our results show how the complexity of the target glycan distribution places functional constraints on the Golgi cisternal number and enzyme specificity.
