论文标题
电化学诱导的pH变化：时间分辨共聚焦荧光显微镜测量和与数值模型的比较
Electrochemically Induced pH Change: Time-Resolved Confocal Fluorescence Microscopy Measurements and Comparison with Numerical Model
论文作者
论文摘要
共聚焦荧光显微镜是一种验证的技术，可以对接近电极的pH变化进行成像。为了完全了解电极过程，需要进行时间分辨的测量，尚未提供。在这里，我们介绍了与共聚焦荧光显微镜的时间分辨pH谱的第一个测量。实验结果与一维反应扩散模型相比有利。这必须到达测量值揭示pH分布中的三维性的程度。还讨论了影响pH测量的特定因素，例如衰减光和染料迁移的作用。该方法进一步应用以揭示在含硫酸盐的电解质中观察到的缓冲效应。此处介绍的工作是为在3D时间分辨pH变化的测量中铺平了使用共聚焦荧光显微镜的方式，例如在气泡附近。
Confocal fluorescence microscopy is a proven technique, which can image near-electrode pH changes. For a complete understanding of electrode processes, time-resolved measurements are required, which have not yet been provided. Here we present the first measurements of time-resolved pH profiles with confocal fluorescence microscopy. The experimental results compare favorably with a one-dimensional reaction-diffusion model; this holds up to the point where the measurements reveal three-dimensionality in the pH distribution. Specific factors affecting the pH measurement such as attenuation of light and the role of dye migration are also discussed in detail. The method is further applied to reveal the buffer effects observed in sulfate-containing electrolytes. The work presented here is paving the way toward the use of confocal fluorescence microscopy in the measurement of 3D time-resolved pH changes in numerous electrochemical settings, for example in the vicinity of bubbles.
