论文标题
COVID-19诊断目标的突变
Mutations on COVID-19 diagnostic targets
论文作者
论文摘要
有效，敏感和可靠的诊断试剂对于打击持续的冠状病毒疾病2019（COVID-19）大流行至关重要。当时没有预防性疫苗或可用于严重急性呼吸综合征冠状动脉综合征2（SARS-COV-2）的特异性药物。如果目前使用的诊断试剂以任何方式破坏，那将是绝对的悲剧。基于截至2020年5月1日收集的7818 SARS-COV-2基因组样品的基因分型，我们透露，基本上所有当前的COVID-19诊断靶标都有突变。我们进一步表明，SARS-COV-2在各种核素（N）基因引物和探针的靶标上具有最具破坏性的突变，不幸的是，这些突出是由世界各地诊断为Covid-19的。我们的发现解释了中国制造的特定诊断试剂严重出了问题。为了了解SARS-COV-2基因是否已突变不均匀，我们计算了所有SARS-COV基因的突变比和突变$ H $索引，这表明N基因是SARS-COV-2基因组中最非保守的基因。我们的发现使研究人员能够针对最保守的SARS-COV-2基因和蛋白质，以设计和开发COVID-19诊断试剂，预防性疫苗和治疗性药物。
Effective, sensitive, and reliable diagnostic reagents are of paramount importance for combating the ongoing coronavirus disease 2019 (COVID-19) pandemic at a time there is no preventive vaccine nor specific drug available for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). It would be an absolute tragedy if currently used diagnostic reagents are undermined in any manner. Based on the genotyping of 7818 SARS-CoV-2 genome samples collected up to May 1, 2020, we reveal that essentially all of the current COVID-19 diagnostic targets have had mutations. We further show that SARS-CoV-2 has the most devastating mutations on the targets of various nucleocapsid (N) gene primers and probes, which have been unfortunately used by countries around the world to diagnose COVID-19. Our findings explain what has seriously gone wrong with a specific diagnostic reagent made in China. To understand whether SARS-CoV-2 genes have mutated unevenly, we have computed the mutation ratio and mutation $h$-index of all SARS-CoV genes, indicating that the N gene is the most non-conservative gene in the SARS-CoV-2 genome. Our findings enable researchers to target the most conservative SARS-CoV-2 genes and proteins for the design and development of COVID-19 diagnostic reagents, preventive vaccines, and therapeutic medicines.
