论文标题
应力诱导的单细胞水平上的鞭毛藻生物发光
Stress-Induced Dinoflagellate Bioluminescence at the Single Cell Level
论文作者
论文摘要
许多海洋鞭毛鞭毛的特征之一是它们的生物发光，它亮起了夜间破裂的波浪或船只prow切成薄片的海水。尽管这些微生物的光生成的内部生物化学已经很好地确定，但流体剪切或机械力触发生物发光的方式仍然很少了解。我们报告了使用局部流体流量或直接注入的海洋鞭毛藻酸盐$ pyrocystis〜lunula $的高速成像，报告了单细胞水平上机械应力与光产生之间关系的控制测量。我们发现粘弹性响应，其中光强度取决于变形的幅度和速率，这与拉伸激活的离子通道的作用一致。现象学模型捕获了实验观测。
One of the characteristic features of many marine dinoflagellates is their bioluminescence, which lights up nighttime breaking waves or seawater sliced by a ship's prow. While the internal biochemistry of light production by these microorganisms is well established, the manner by which fluid shear or mechanical forces trigger bioluminescence is still poorly understood. We report controlled measurements of the relation between mechanical stress and light production at the single-cell level, using high-speed imaging of micropipette-held cells of the marine dinoflagellate $Pyrocystis~lunula$ subjected to localized fluid flows or direct indentation. We find a viscoelastic response in which light intensity depends on both the amplitude and rate of deformation, consistent with the action of stretch-activated ion channels. A phenomenological model captures the experimental observations.
