论文标题
与共聚焦相显微镜的组织形态的高分辨率标签成像
High-resolution label-free imaging of tissue morphology with confocal phase microscopy
论文作者
论文摘要
无标签的成像方法通过替换染料的当前染色实践来通过定量的光学测量来可视化组织形态，以简化和增强组织病理学评估。定量相成像（QPI）以可见/紫外线的方式运行，因此提供了与当前实践相匹配的分辨率。在这里，我们介绍并演示了用于组织切片的无标签成像的共共聚焦QPI，并评估了其对手动组织病理学检查的效用。成像癌性和正常的邻近人乳房和前列腺，我们表明组织结构组织可以通过与常规H＆E染色相当的高空间细节来解决。发现我们的共聚焦QPI图像没有光环，在QPI中解决了这个常见问题。我们进一步描述并应用了一个基于有限差分时间域（FDTD）计算的虚拟成像系统，以定量地比较共焦点与广阔的QPI方法，并使用数值组织幻象来探索性能限制。
Label-free imaging approaches seek to simplify and augment histopathologic assessment by replacing the current practice of staining by dyes to visualize tissue morphology with quantitative optical measurements. Quantitative phase imaging (QPI) operates with visible/UV light and thus provides a resolution matched to current practice. Here we introduce and demonstrate confocal QPI for label-free imaging of tissue sections and assess its utility for manual histopathologic inspection. Imaging cancerous and normal adjacent human breast and prostate, we show that tissue structural organization can be resolved with high spatial detail comparable to conventional H&E stains. Our confocal QPI images are found to be free of halo, solving this common problem in QPI. We further describe and apply a virtual imaging system based on Finite-Difference Time-Domain (FDTD) calculations to quantitatively compare confocal with wide-field QPI methods and explore performance limits using numerical tissue phantoms.
