ISO INTERNATIONAL STANDARD 23040 First edition 2021-12 Marine environment impact assessment (MEIA) Specification for marine sediments in seabed areas Survey of interstitial biota Evaluation de I'impact environnemental marin Spécifications relatives aux sediments marins dans les zones de fonds marins Etude du biote interstitiel Reference number IS0 23040:2021(E) ISO @IS02021 IS0 23040:2021(E) COPYRIGHT PROTECTED DOCUMENT @ IS02021 All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may the internet or an intranet, withoutpriorwrittenpermission.Permission can be requested from eitherIso at the addressbelow or Iso's member body in the country of the requester. ISO copyright office CP 401 · Ch. de Blandonnet 8 CH-1214Vernier,Geneva Phone: +4122 749 0111 Email: [email protected] Website: www.iso.org PublishedinSwitzerland i @ IS0 2021 - All rights reserved IS0 23040:2021(E) Contents Page Foreword ..viii Introduction .ix 1 Scope. 1 2 Normative references .1 3 Terms and definitions. .1 4 General .2 4.1 Technical design. .2 4.2 Basic recommendations for the surveys 2 4.2.1 Survey object.. 2 Auxiliary parameters 3 4.2.3 Recommendations for the sampling equipment 3 4.2.4 Auxiliary equipment on board ship 3 4.2.5 Sampling method and scope of application .3 4.3 Sampling 3 4.3.1 Sediment sampling. .3 4.3.2 Trawl sampling. 3 4.3.3 Water sampling 4 4.3.4 Records 4 4.4 Sample analysis. .4 4.4.1 Sample treatment 4 4.4.2 Sample measurement 4 4.4.3 Sample treatment and storage. 4 4.4.4 Sample identification and enumeration .4 4.4.5 Sample data analysis 4 4.5 Basic recommendations of data organization. .5 4.5.1 Organization of data. .5 4.5.2 Data archiving, acceptance and achievements appraisal 5 4.6 Surveyresults. 5 4.6.1 Original records .5 4.6.2 Maps or drawings. 5 4.6.3 Investigation report .5 4.7 Data archiving .6 4.8 Program and quality control .6 5 Survey of the sediment .6 5.1 Principle .6 5.2 General provisions .7 5.3 Collection and preservation of the samples 7 5.4 Measurement of environmental factors 7 5.5 Measurement of the age of the sediments. 7 5.6 Measurement of the contents of heavy metals, organic pollutants and oils in the sediments .7 5.7 Measurement of the grain size of the sediments 7 5.8 Analysis ofthemineral compositions inthe sediments. 8 5.9 Classificationofthesubstratetypeinoffshoresediments .8 5.10 Classificationofthedeep-seasediments 8 5.11 Survey of the biological components in the sediments .8 5.11.1 Summary of the methods. .8 5.11.2 Technical recommendations. 8 5.11.3 Collection and preservation of the samples .9 5.12 Organization of data. .9 6 Survey of foraminifera .9 6.1 Principle 9 @ IS0 2021 - All rights reserved ii IS0 23040:2021(E) 6.2 General provisions 9 6.3 Collection and preservation of the samples 9 6.3.1 Sampling and sample processing. 9 6.3.2 Collection and preservation of the sedimentary tests ..10 6.3.3 Collection and preservation of living foraminifera. ..10 6.4 Tools and reagents . .10 6.5 Processing and analysis of the samples. ..10 6.5.1 Numbering and weighing the crystallizing dish or beaker. ..10 6.5.2 Drying and weighing the sediment sample 6.5.3 Sample soaking. ..11 6.5.4 Washing and drying .11 6.5.5 Suspension and concentration. .11 6.5.6 Bottling and sealing... ..11 6.5.7 Specimen preparation and analysis ..11 1 Survey of ostracoda ..12 7.1 Principle. .12 7.2 General provisions. 7.3 Collection and preservation of the samples .12 7.3.1 Sampling and sample processing. .12 7.3.2 Collection and preservation of dead ostracod tests ..13 7.3.3 Collection and preservation of living ostracods 7.4 Tools and reagents ..13 7.5 Processing and analysis of the samples .13 7.5.1 Numbering and weighing the crystallizing dish or beaker. .13 7.5.2 Drying and weighing... .13 7.5.3 Sample soaking ..14 7.5.4 Washing and drying. .14 7.5.5 Suspension and concentration. ..14 7.5.6 Bottling and sealing. .14 7.5.7 Microscopic examin