BS IS0 21766:2021 BSl Standards Publication Tobacco and tobacco products Determination of tobacco-specific nitrosamines in tobacco products Method using LC-MS/MS bsi. BSISO21766:2021 BRITISH STANDARD National foreword This British Standard is the UK implementation of IS0 21766:2021. It supersedes BSIS0 21766:2018, which is withdrawn. The UK participation in its preparation was entrusted to Technical Committee AW/4o, Tobacco and tobacco products. A list of organizations represented on this committee can be obtained on requestto its committeemanager. This publication does not purport to include all the necessary provisions ofa contract.Users areresponsiblefor its correct application. @TheBritishStandards Institution2021 Published byBSI StandardsLimited2021 ISBN9780539152869 ICS 65.160 Compliance with a British Standard cannot confer immunity from legal obligations. This British Standard was published under the authority of the Amendments/corrigendaissuedsincepublication Date Textaffected BS ISO 21766:2021 INTERNATIONAL ISO STANDARD 21766 Second edition 2021-02-03 Tobacco and tobacco products Determination of tobacco-specific nitrosamines in tobacco products Method using LC-MS/MS Tabacetproduitsdutabac-Dosagedesnitrosaminesspécifiquesdu tabacdanslesproduitsdutabac-MethodeparCL-SM/SM Reference number ISO 21766:2021(E) ISO @ ISO 2021 BSIS021766:2021 IS021766:2021(E) COPYRIGHTPROTECTEDDOCUMENT ISo 2021,Published in Switzerland All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying,or posting on the internet or an intranet, without prior written permission. Permission can be requested from either ISo at the address below or ISo's member body in the country of the requester. ISO copyright office Ch. de Blandonnet 8. CP 401 CH-1214 Vernier, Geneva, Switzerland Tel. +41 22 749 01 11 Fax +41 22 749 09 47 [email protected] www.iso.org i @ IS0 2021 - All rights reserved BSISO21766:2021 IS021766:2021 Contents Page Foreword ..iv Introduction. .. 1 Scope. 2 Normative references 3 Terms and definitions 4 Principle ..1. 5 Reagents. ..1 6 Apparatus. .2 6.4 Autosampler vials (amber). .3 7 Preparation. .3 7.1 Preparation of glassware .3 7.2 Preparation of solutions 3 7.2.1 Extraction solution,1o0mMammoniumacetatein water. 3 7.2.2 HPLCMobilePhaseA:Water,resistivity≥18,2M2-cmat25°C 3 7.2.3 HPLC MobilePhaseB:0,1%acetic acid inmethanol 3 7.3 Preparation of standards. 3 .3 7.3.1 General. 7.3.2 Preparation of internal standard solutions 4 7.3.3 Preparation of calibration standard solutions .4 8 Sampling 8.1 General .5 8.2 Sample preparation ..5 8.3 Sample extraction. .5 9 Sample analysis .6 9.1 General ..6 9.2 SuggestedHPLCparameters .6 9.3 MS/MSparameters. .6 9.3.1 General .6 9.3.2 Quantification and qualification transitions .7 .7 9.4 System suitability 9.5 Calibration. .7 9.6 Calculation 10 Repeatabilityandreproducibility ..8 11 Test report ..12 Annex A (informative) Sample clean-up using solid phase extraction (SPE) ..13 AnnexB (informative)Examples oftypical chromatograms. ..15 Bibliography .16 @IS0 2021-All rights reserved ili BSISO21766:2021 IS0 21766:2021 Foreword Iso(theInternational OrganizationforStandardization)isaworldwidefederationofnationalstandards bodies (Iso member bodies). The work of preparing International Standards is normally carried out throughIsotechnical committees.Eachmemberbody interested inasubjectforwhichatechnical committee has been established has the right to be represented on that committee. International organizations,governmental and non-governmental, in liaison withIso,also take part in the work. ISoO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of electrotechnicalstandardization The procedures used to develop this document and those intended for its further maintenance are described in the Iso/IEC Directives, Part 1. In particula