BS ISO 18337:2015 BSl Standards Publication Surface chemical analysis - Surface characterization - Measurement of the lateral resolution of a confocal fluorescence microscope bsi. ..making excellenCe a habit. BS ISO 18337:2015 BRITISH STANDARD National foreword This British Standard is the UK implementation of ISO 18337:2015. The UK participation in its preparation was entrusted to Technical CommitteeCll/60, Surface chemical analysis. A list of organizations represented on this committee can be obtained on request to its secretary. This publication does not purport to include all the necessary provisions of a contract. Users are responsible for its correct application. @The British Standards Institution 2015.Published by BSI Standards Limited 2015 ISBN9780580811326 ICS 71.040.40 Compliance witha BritishStandard cannot conferimmunityfrom legal obligations. This British Standard was published under the authority of the Standards Policy and Strategy Committee on 30 June 2015. Amendments issued since publication Date Text affected BS ISO 18337:2015 ISO 18337 INTERNATIONAL STANDARD First edition 2015-06-15 Surface chemical analysis Surface characterization Measurement of the lateral resolution of a confocal fluorescence microscope Analysechimiquedes surfacesCaractérisationdessurfaces Mesurage de la résolution laterale d'un microscope confocal d fluorescence TSO ISOReggceoug bey @IS02015 BSISO18337:2015 IS018337:2015(E) COPYRIGHTPROTECTEDDOCUMENT ISO2015, Published in Switzerland AHbyghty rasansedlalestronicotherwipenifiedlaniclaindfiuthisgpplbbicatbpyimgyoltqnsthgintprndtioedntnttiaet) otineamyisovithout prior written permission. Permission can be requested from either ISO at the address below or ISo's member body in the country of the requester. fsodecparibh Aeics 3·CP401 CH-1214 Vernier, Geneva, Switzerland Tel.+41 22 749 0111 Fax +41 22 749 09 [email protected] 47 www.iso.org ii @ IS0 2015 - All rights reserved BS ISO18337:2015 IS018337:2015(E) Contents Page Foreword iv Iptroduction. Scope 1 2 Terms and definitions 3 Symbols and abbreviated terms 4 General 1 4.1 Background information 4.2 Types of CFM operation 2 4.2.1 General Stage scanning CFM 4.2.3 Laser scanning CFM .2 4.2.4 Spinning disk CFM 2 arametersGethataffect the lateral resolution.of a CFM 4.3 4.3.2 Objectivelens 3 4.3.3 Detectionpinholesizeandfocal lengthofthetubelens 3 4:3:5 Solinzatifohafthsuarer hf dasrallumination beam 4.3.6 Excitationandemissionwavelengths 5 4.3.7 Imagecontrast 3 Measuringthelateralresolutionbyimaginga smallobject 3 5.1 Background information 3 5.2 Selection ofthe sample and samplerequirements 5.4 Seatingcothectionparaheterpaiatysisperating.the.instrument 5.4.1 Selecting a proper spot. 5 5.4.2 Extracting a line profile using band-average process 5 5.5 Recording the data. 6 Annex A (informative) Sample preparation and example of data and analysis .7 Bibliography 10 @ IS0 2015 - All rights reserved iii BS ISO 18337:2015 IS018337:2015(E) Foreword Iso (the International Organization for Standardization) is a worldwide federation of national standards thundiegh(IsO tecamicet codbiesfthsewofkaf ptapbeingbodyInitteresitedailhStandebjdsti soowhidhycarrtiechaital committeehasbeen established hastherighttoberepresentedonthatcommittee.International organizations, governmental and non-governmental, in liaison with Iso, also take part in the work. ISO collaborates closely with the electrotechnical standardization.International Electrotechnical Commission (IEC)on allmatters of The procedures used to develop this document and those intended for its further maintenance are diferneatyphes ofso/cdBiretiyes SRutia benoted Thisah&different daassrvsiteriaccordance woh the editorialrulesof theIsO/IEC Directives,Part2 (seewww.iso.org/directives). Rttentionrights.if&wrshtahf hetpelsibilithat somsible fbe fdlemtfryting anyafostrh aatenteay hgtbe Detaisfubfe ctany patentrightsidentifiedduringthede the Iso list of patent declarations received (see www.iso.org/patents).