BS ISO 16778:2015 BSI Standards Publication Water quality Calanoid copepod early-life stage test with Acartia tonsa bsi. ..making excellenCe a habit. BS ISO 16778:2015 BRITISH STANDARD National foreword This British Standard is the UK implementation of ISO 16778:2015. The UK participation in its preparation was entrusted to Technical Committee EH/3/5, Biological Methods. A list of organizations represented on this committee can be obtained on request to its secretary. This publication does not purport to include all the necessary provisions of a contract. Users are responsible for its correct application. The British Standards Institution 2015 Published by BSIStandards Limited 2015 ISBN9780580749179 ICS 13.060.70 CompliancewithaBritishStandard cannotconferimmunityfrom legal obligations. This British Standard was published under the authority of the Standards Policy and Strategy Committee on 30 June 2015. Amendments/corrigendaissuedsincepublication Date Text affected ISO 16778 INTERNATIONAL STANDARD First edition 2015-06-15 Water quality - Calanoid copepod early-life stage test with Acartia tonsa Qualitéde I'eau-Essai aux premiers stades de la viede copepodes calanoides avec Acartia tonsia EIIE TSO @IS02015 BSISO16778:2015 IS016778:2015(E) COPYRIGHTPROTECTEDDOCUMENT ISO2015, Published in Switzerland AHbyghty rasansedlalestronicotherwipenifiedlaniclaindfiuthisgpplbbicatbpyimgyoltqnsthgintprndtioedntnttiaet) otineamyisovithout prior written permission. Permission can be requested from either ISO at the address below or ISo's member body in the country of the requester. fsodecparibh Aeics 3·CP401 CH-1214 Vernier, Geneva, Switzerland Tel.+41 22 749 0111 Fax +41 22 749 09 [email protected] 47 www.iso.org ii @ IS0 2015 - All rights reserved BS ISO16778:2015 IS0 16778:2015(E) Contents Page Foreword Scope 1 2 Normative references .1 3 Terms and definitions 1 4 Principle .2 5 Apparatus. 2 6 Reagents .3 6.1 Water .3 6.2 Culture and test media 3 7 Test organism. 8 Procedure. 4 8.1 Preparation ofculture medium .4 8.2 Choiceoftest concentrations 4 Preparation of test substangglutions'stock'solution 8:4 8.5 Incubation/Exposure .5 8.5.1 Test organisms and loading ..5 8:5:2 earvfbrfatchjnefatio (Early-life stages development) 6 5 8.5.4 ..6 Duration. 6 8.5.5 Handling of test vessels. 8:5:9 Feeding 6 Light andtemperature 8.5.8 Aeration 6 8.5.9 Dilutionwaterandtestsolutionsrenewal oraddition 8.6 Measurements/Observations. 8.6.1 Concentration ofthetest substance 8.6.2 Physical-chemical parameters .. 9 oxygen,pH,salinity,andtemperature Validity criteria 8 10 Expression of results .8 11 Denotion of results 9 12 Interpretation of results. 13 Reference substance .9 14 Test report. 9 AnnexA (informative)Definedcultureandtestmedia 11 AnnexBxanplatiaffsyeiagraetdasalarealderelpngenhtestherrluliferstaartestith Acartiatonsa .13 AnnexC (informative) Samplingfor chemical analyses .17 AnnexD (informative)Lugol's solution .18 Annex E (informative) Data collection sheetfor Acartiatonsa early-life stagetest .19 AnnexF(informative)Calculations .21 AnnexG(informative)CulturingofAcartiatonsa .23 @ IS0 2015 - All rights reserved iii BS ISO 16778:2015 IS0 16778:2015(E) AnnexH (informative) Specific details on secondarysexual characteristics for Acartiatonsa .28 Annex I (informative) General safety precautions and techniques for handling .30 environmentalsamples. Bibliography .36 iv @ IS0 2015 - All rights reserved BS ISO 16778:2015 IS0 16778:2015(E) Foreword Iso (the International Organization for Standardization) is a worldwide federation of national standards thodiegh(IsO tectmiset boabiesjtahsewofkdf pmtepaeingbodyInitteresitedaihStandtbjdstisorowhidlycarrtiechmital committeehas been established hastherighttoberepresentedonthat committee. International organizations, governmental and non-governmental, in liaison with Iso, also take part in the work. ISO collaborates closely with the electrotechnical standardization.International Electrotechnical Commission (IEC)o