ISO INTERNATIONAL STANDARD 14718 First edition 1998-12-15 Animal feeding stuffs Determination of aflatoxin B content of mixed feeding stuffs Method using high-performance liquid chromatography Aliments des animaux - Détermination de la teneur en aflatoxine B dans les aliments composés Methode par chromatographie liquide a haute performance ISO Referencenumber ISO 14718:1998(E) y IHS under ted without license from IHS Not for Resale ISO 14718:1998(E) Foreword IsO (the International Organization for Standardization) is a worldwide federation of national standards bodies (isO the right to be represented on that committee. International organizations, governmental and non-governmental, in liaison with ISO, , also take part in the work. isO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization. International Standards are drafted in accordance with the rules given in the ISO/lEC Directives, Part 3. Draft International Standards adopted by the technical committees are circulated to the member bodies for voting Publication as an International Standard requires approval by at least 75 % of the member bodies casting a vote. International Standard ISO 14718 was prepared by Technical Committee ISO/TC 34, Agricultural food products, Subcommittee SC 10, Animal feeding stuffs. Annexes A and B of this International Standard are for information only @ISO1998 All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized in any form or by any means, electronic International Organization for Standardization Case postale 56 · CH-1211 Geneve 20 · Switzerland Internet [email protected] Printed in Switzerland Not for Resale ISO 14718:1998(E) INTERNATIONAL STANDARD @ISO Animal feeding stuffs Determination of aflatoxin B, content of mixed feeding stuffs Method using high-performance liquid chromatography 1 Scope determination of aflatoxin B, content of animal feeding stuffs including those containing citrus pulp. The lower limit of determination is 1 μg/kg. NOTE 1 This International Standard may be applicable for the determination of the aflatoxin B, content of a number of raw materials and straight feeding stuffs such as corn gluten, groundnut, palm kernel, copra, citrus pulp, tapioca, soya bean, rice bran,pollard,rape seed,nigerseed and cotton seed (see references[1] and[2]).These materials were,however,not includeo in :the collaborative testing of the method. NOTE 2 This International Standard may also be applicable for the determination of the content of the sum of the aflatoxins By, B2, G, and Gz. However, the method has not been validated for this parameter by collaborative testing. 2 Normative reference The following normative document contains provisions which, through reference in this text, constitute provisions of this International Standard. For dated references, subseqent amendments to, or revisions of, this publication do not apply. However, parties to agreements based on this International Standard are encouraged to investigate the the latest edition of the normative document referred to applies. Members of IEC and ISO editions maintain registers of currently valid International Standards. IsO 6498:1998, Animal feeding stuffs Preparation of test sample. 3 Principle The sample is extracted with chloroform. The extract is filtered and an aliquot portion is purified on a Florisil@1) cartridge and a C18 cartridge. The final separation and determination is achieved by high-performance liquid chromatography (HPLC) using a reverse-phase C18 column, followed by post-column derivatization with iodine or bromine, and fluorescence detection. 4 Reagents and materials Use only reagents of recognized analytical grade. 1) Florisil@ is the trade-name of a commercially available product. This information is given for the convenience of users of this International Standard and does not constitute an endorsement by IsO of this product. Eq