ISO INTERNATIONAL STANDARD 1241 Second edition 1996-12-15 ANsi Internat Doc Sec Essential oils Determination of ester values, before and after acetylation, and evaluation of the contents of free and total alcohols Huiles essentielles -Determination de I'indice d'ester, avant et apres acétylation, et évaluation de la teneur en alcools libres et en alcools totaux ISO Reference number ISO 1241:1996(E) No reproduction or networking permited without license from IHS Not for Resale ISO1241:1996(E) FOREWORD IsO (the International Organization for Standardization) is a worldwide federation of national standards bodies (lsO member bodies).The work of preparing International Standards is normally carried out through isO technical committees. Each member body interested in a subject for which a technical committee has been established has the right to be represented on that committee. International organizations, governmental and non-governmental, in liaison with isO, also take part in the work. ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization. Draft International Standards adopted by the technical committees are circulated to the member bodies for voting. Publication as an International Standard requires approval by at least 75 % of the member bodies casting a vote. International Standard ISO 1241 was prepared by Technical Committee 1SO/TC 54, Essential oils. This second edition cancels and replaces the first edition (IsO 1241:1980), which has been technically revised. @ISO1996 or utilized in any form or by any means, electronic or mechanical, including photocopying and microfilm,without permission in writing from the publisher. InternationalOrganizationforStandardization Casepostale56CH-1211Geneve20.Switzerland Internet [email protected] X.400 C=ch; a=400net; p=iso; 0=isocs; S=central Printed in Switzerland ii No reproduction or networking permited without license from IHS Not for Res INTERNATIONAL STANDARD ISO ISO 1241:1996(E) Essential oils --- Determination of ester values, before and after acetylation, and evaluation of the contents of free and total alcohols 1 SCOPE This International Standard specifies a method to evaluate the contents of free alcohols and total alcohols in essential oils by determination of ester values before and after acetylation by acetic anhydride in the presence of sodium acetate. The method is not applicable to essential oils containing appreciable quantities of tertiary alcohols (for example linalol and terpineols), which would not be completely acetylated. NOTE: For these essential oils the method given in ISO 37941) should be used. The method is not applicable to essential oils containing appreciable quantities of phenols lactones, aldehydes or enolysable ketones, which would be acetylated in addition to free alcohols. 2 NORMATIVEREFERENCES The following standards contain provisions which, through reference in this text, constitute provisions of this International Standard. At the time of publication, the editions indicated were valid. All standards are subject to revision, and parties to agreements based on this International Standard are encouraged to investigate the possibility of applying the most recent editions of the standards indicated below. Members of iEC and Iso 'maintain registers of currently valid International Standards. Iso 212:1973, Essential oils — Sampling. IsO 356:1996, Essential oils --- Preparation of test sample. ISO 385-1:1984, Laboratory glassware - Burettes Part 1: General requirements. Is0709:1980,Essentialoils—Determinationofestervalue. 1) ISO 3794:1976, Essential oils (containing tertiary alcohols) - Estimation of free alcohols content by determination of estervalueafteracetylation. 1 Noreproductionor networking pemited without iensefrom IHS Not for Resale