ISO INTERNATIONAL STANDARD 3890-2 IDF 75-2 Second edition 2009-10-15 Milk and milk products Determination of residues of organochlorine compounds (pesticides) - Part 2: Test methods for crude extract purification and confirmation Lait et produits laitiers Détermination des résidus de composés organochloreés (pesticides) Partie 2: Methodes d'essai pour la purification des extraits bruts et tests de confirmation Reference numbers ISO 3890-2:2009(E) So IDF 75-2:2009(E) @ ISO and IDF 2009 HS under I without license from IHS Not for Resale ISO 3890-2:2009(E) IDF 75-2:2009(E) PDF disclaimer This PDF file may contain embedded typefaces. In accordance with Adobe's licensing policy, this file may be printed or viewed but shall not be edited unless the typefaces which are embedded are licensed to and installed on the computer performing the editing. In downloading this file, parties accept therein the responsibility of not infringing Adobe's licensing policy. Neither the ISo Central Secretariat nor the IDF accepts any liability in this area. Adobe is a trademark of Adobe Systems Incorporated. Details of the software products used to create this PDF file can be found in the General Info relative to the file; the PDF-creation and IDF national committees. In the unlikely event that a problem relating to it is found, please inform the ISO Central Secretariat at the address given below. COPYRIGHT PROTECTED DOCUMENT @ ISO and IDF 2009 All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized in any form or by any means, address below. ISO copyright office International Dairy Federation Case postale 56 . CH-1211 Geneva 20 Diamant Building·Boulevard Auguste Reyers 80.B-1030 Brussels Tel. + 41 22 749 01 11 Tel. + 32 2 733 98 88 Fax + 41 22 749 09 47 Fax + 32 2 733 04 13 E-mail [email protected] E-mail [email protected] Web www.iso.org Web www.fil-idf.org Published in Switzerland Copyrgh Interatinal Oorganization for Standardization @ISOand IDF2009-All rights reserved vided by IHS under Not for Resale ISO 3890-2:2009(E) IDF 75-2:2009(E) Contents Page Foreword 1 Scope. 2 Normative references.. 3 Method A: Liquid-liquid partitioning with acetonitrile and clean-up on a Florisil column .... 4 Method B: Liquid-liquid partitioning with dimethylformamide (DMF) and clean-up on an aluminacolumn. 5 Method C: Liquid-liquid partitioning with dimethylformamide (DMF) and clean-up on a Florisil1) column... .6 Method D: Column chromatography on aluminium oxide of precisely defined activity ... 7 Method E: Column chromatography on alumina column, 8 Method F: Column chromatography on partially deactivated Florisil1). 9 Method G: Column chromatography on partially deactivated silica gel... 10 Method H: Gel-permeation chromatography...... 11 Confirmatory tests and additional clean-up procedure 12 Additional clean-up procedure 27 Bibliography... ii by IHS unde nse from IHS Not for Resale