ISO/TS TECHNICAL SPECIFICATION 18220 First edition 2016-07-01 Water quality - Larval development test with the harpacticoid copepod Nitocra spinipes Qualite de I'eau - Essai de développement larvaire avec le copépode harpacticoide Nitocra spinipes Reference number ISO/TS 18220:2016(E) ISO International Organization for Standardization @IS02016 nitted without license from IHS IS0/TS 18220:2016(E) COPYRIGHT PROTECTEDDOCUMENT IS0 2016, Published in Switzerland All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address below or ISO's member body in the country of the requester. ISO copyright office Ch. de Blandonnet 8 . CP 401 CH-1214 Vernier, Geneva, Switzerland Tel. +41 22 749 01 11 Fax +41 22 749 09 47 [email protected] www.iso.org Internatinai br DrganizationforStandardization networking permited without license from IHS IS0/TS 18220:2016(E) Contents Page Foreword ..iv Introduction. ..V 1 Scope. ..1 2 Terms and definitions 3 Principle 4 Reagents. ..2 5 Cultivation .3 5.1 Test organism .3 5.2 Algae for feeding ..3 6 Apparatus ..3 7 Procedure ..4 7.1 Production of naupli to be used in test 7.2 Choice of test concentrations 4 7.2.1 Hypothesis testing. .5 7.2.2 Regression analysis.. 5 7.3 Preparation of solutions to be used in test 5 7.3.1 Stocksolution .5 7.3.2 Test solutions 5 7.4 Startoftest .6 7.5 Incubation/exposure. .6 7.6 Maintenance .6 7.7 Measurements/observations ..6 7.7.1 Mortality .6 7.7.2 Larval development ratio (LDR), .7 7.7.3 Physical-chemical parameters - oxygen, pH and salinity .7 7.7.4 Concentration of the test substance. 8 Validity criteria .7 9 Evaluation of results ..8 9.1 Calculation of results .8 9.2 Expression of results ..8 9.3 Interpretation of results ..8 10 Reproducibility .8 11 Test report. .8 Annex A (informative) Biology and cultivation of Nitocra spinipes ..10 Annex B (informative) Nitocra spinipes larval development ratio ..15 Annex C (informative) Calculations ..17 Bibliography .19 ii d without license from IHS