ISO/TR TECHNICAL REPORT 6579-3 First edition 2014-07-15 Microbiology of the food chain Horizontal method for the detection, enumeration and serotyping of Salmonella Part 3: Guidelines for serotyping of Salmonella spp. Microbiologie de la chaine alimentaireMethodehorizontalepour la recherche, le dénombrement et la sérotypie des Salmonella - Partie 3: Lignes directrices pour la sérotypie des Salmonella spp. Reference number IS0/TR 6579-3:2014(E) LSO @ IS0 2014 I without license from IHS Not for Resale IS0/TR 6579-3:2014(E) COPYRIGHTPROTECTEDDOCUMENT ISO2014 All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address below or ISO's member body in the country of the requester. ISOcopyrightoffice Case postale 56:CH-1211 Geneva 20 Tel. + 41 22 749 01 11 Fax +4122 749 09 47 E-mail [email protected] Web www.iso.org Published in Switzerland @ IS0 2014 - All rights reserved No reproductic networking permited without license from IHS Not for Resale IS0/TR 6579-3:2014(E) Contents Page Foreword ..iv Introduction ..V 1 Scope. ..1 2 Normative references 3 Terms and definitions ..1 4 Principle ..2 5 Culture media and sera .2 5.1 General .2 5.2 Culture media and reagents .2 5.3 Antisera .2 6 Apparatus. .2 7 Sample. .3 8 Taxonomy of Salmonella .3 8.1 General .3 8.2 Nomenclature. .3 8.3 Biochemical characteristics .4 8.4 Antigenic characteristics ..5 9 Procedure for Salmonella serotyping .7 9.1 General 7 9.2 Example procedure for serotyping five public health-related Salmonella serovars .7 10 Quality control .11 11 Reporting .11 Annex A (informative) Composition and preparation of culture media and reagents .13 Annex B (informative) Examples of procedures for serotyping an unknown Salmonella isolate....20 Annex C (informative) Biochemical tests .24 Annex D (informative) Schematic overview for serotyping five important public-health related Salmonella serovars .26 Annex E (informative) Microtitre plate method for serotyping Salmonella spp .27 Annex F (informative) Examples of procedures for phase inversion ..29 Bibliography .32 iii ut license from IHS Not for Resale