TECHNICAL ISO/TR REPORT 17622 First edition 2015-11-01 Molecular biomarker analysis SSR analysis of sunflower Analyse moléculaire de biomarqueurs - Méthode d'analyse SsR sur le tournesol Reference number IS0/TR 17622:2015(E) SO International Organization for Standardization ZHEJIANG INST OF STANDARDIZATION C15956617 IS02015 vided by IHS under itted without license from IHS IS0/TR 17622:2015(E) COPYRIGHTPROTECTEDDOCUMENT IS02015,PublishedinSwitzerland All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address below or ISO's member body in the country of the requester. ISO copyrightoffice Ch. de Blandonnet 8 . CP 401 CH-1214 Vernier, Geneva, Switzerland Tel. +41 22 749 01 11 Fax +41 22 749 09 47 [email protected] www.iso.org Intenationaibr PrganizationforStandardization icensee-ZHEJIANG INST OF STANDARDIZoISQ s1.5 - All rights reserved etworking permitted without license from IHS IS0/TR 17622:2015(E) Contents Page Foreword ..iv Introduction. ..V 1 Scope.. ..1 2 Principle ..1 3 Consumables and equipment 4 Procedure .1 4.1 Sample preparation .1 4.2 DNA Extraction and Quantification .2 4.3 PCR amplification. .2 5 Established list of SsR markers for sunflower hybrid conformity testing .3 5.1 Characteristics of the SSRs. 5.2 SSR primer sequences. 4 5.3 Observed SSR profiles of sunflower lines .5 Bibliography 6 iii ee=ZHEJIANG INST OF STANDARDIZATION C1 5956617 vided by IHS under ithout license from IHS