ISO INTERNATIONAL STANDARD 29200 First edition 2013-09-01 Soil quality Assessment of genotoxic effects on higher plants Vicia faba micronucleus test Qualite du sol - Evaluation des effets genotoxiques sur les vegetaux supérieurs -Essai des micronoyaux sur Vicia faba Reference number IS0 29200:2013(E) LSO IS02013 HS under ted without license from IHS Not for Resale IS0 29200:2013(E) COPYRIGHTPROTECTEDDOCUMENT @ IS0 2013 All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized otherwise in any form written permission. Permission can be requested from either ISO at the address below or ISO's member body in the country of the requester. ISO copyright office Case postale 56:CH-1211 Geneva 20 Tel. + 41 22 749 01 11 Fax + 41 22 749 09 47 E-mail [email protected] Web www.iso.org Published in Switzerland @ IS0 2013 - All rights reserved No reproductic networking permited without license from IHS Not for Resale IS0 29200:2013(E) Contents Page Foreword ..iv Introduction. ..V 1 Scope. .1 2 Normative reference ..1 3 Terms and definitions ..1 4 Principle ..2 5 Plants, test equipment and reagents .2 5.1 Equipment. .2 5.2 Test organism .2 5.3 Reference substance. .2 5.4 Reagents. .3 6 Protocols .3 6.1 Preparation of the soil to be tested .3 6.2 Preparation of the seeds .4 6.3 Conducting of the test .5 6.4 Test environment. .6 6.5 Cell preparation .6 7 Assessment of the results .8 7.1 Presentation of the data ..8 7.2 Statistical analysis ..8 7.3 Interpretation of the results ..8 8 Validity criteria ..8 9 Test report .8 Annex A (informative) Composition of Hoagland's medium .10 Annex B (informative) Testing chemicals added to soils. ..11 Annex C (informative) Results of the interlaboratory test conducted within the framework of NFT90-327 12 Annex D (informative) Results of the interlaboratory test conducted on the reference substance and an industrial contaminated soil .14 Bibliography .18 iii se with ISt thout license from IHS Not for Resale