ISO INTERNATIONAL STANDARD 21150 Secondedition 2015-12-01 Cosmetics Microbiology Detection of Escherichia coli CosmétiguesMicrobiologie-Détection d'Escherichia coli Reference number IS0 21150:2015(E) ISO @IS02015 IS0 21150:2015(E) COPYRIGHTPROTECTEDDOCUMENT @ IS0 2015, Published in Switzerland All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address below or ISo's member body in the country of the requester. ISO copyright office Ch. de Blandonnet 8 . CP 401 CH-1214 Vernier, Geneva, Switzerland Tel.+4122 749 0111 Fax +4122 749 09 47 [email protected] www.iso.org @ IS0 2015 - All rights reserved Copvriaht Inte IS0 21150:2015(E) Contents Page Foreword ..iv Introduction. ..V 1 Scope. ..1 2 Normative references ..1 3 Terms and definitions 4 Principle .2 5 Diluents and culture media .2 5.1 General .2 5.2 Diluent for the bacterial suspension (tryptone sodium chloride solution) .3 5.2.1 General .3 5.2.2 Composition. .3 5.2.3 Preparation. 5.3 Culture media. .3 5.3.1 General .3 5.3.2 Agar medium for the suitability test (see Clause 11) [soybean-casein digest agar medium (SCDA) or tryptic soy agar (TSA)) ..3 5.3.3 Enrichment broth. .4 5.3.4 Selective agar medium for isolation of Escherichia coli. .4 5.3.5 Selective agar medium for confirmation of Escherichia coli ..5 6 Apparatus and glassware ..6 7 Strains of microorganisms ..6 8 Handling of cosmetic products and laboratory samples ..6 9 Procedure ..6 9.1 Generalrecommendation ..6 9.2 Preparation of the initial suspension in the enrichment broth .6 9.2.1 General . 6 9.2.2 Water-miscible products .7 9.2.3 Water-immiscible products .7 9.2.4 Filterable products. .7 9.3 Incubation of the inoculated enrichment broth .7 9.4 Detection and identification of Escherichia coli .7 9.4.1 Isolation 1 9.4.2 Identification of Escherichia coli 7 10 Expression of the results (detection of Escherichia coli) 11 Neutralization of the antimicrobial properties of the product .8 11.1 General. ..8 11.2 Preparation of inoculum ..8 11.3 Suitability of the detection method. .8 11.3.1 Procedure 11.3.2 Interpretation of suitability test results .9 12 Test report .9 Annex A (informative) Other enrichment broths ..10 Annex B (informative) Neutralizers of antimicrobial activity of preservatives and rinsing liquids ..13 Bibliography ..14 inta adardzaon rights reserved iii