ISO INTERNATIONAL STANDARD 21148 Second edition 2017-06 Cosmetics Microbiology General instructions for microbiological examination CosmétiquesMicrobiologieInstructions générales pour les examens microbiologiques Reference number IS0 21148:2017(E) International Organization for Standardization @ IS0 2017 =ZHEJIANG INST OF STANDARDIZATION C15956617 vided by IHS underI Not for Resale, 2017/8/21 02:13:01 itted without license from IHS IS0 21148:2017(E) COPYRIGHTPROTECTEDDOCUMENT IS0 2017, Published in Switzerland All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized otherwise in any form written permission. Permission can be requested from either ISO at the address below or ISO's member body in the country of the requester. ISO copyrightoffice Ch. de Blandonnet 8 . CP 401 CH-1214 Vernier, Geneva, Switzerland Tel. +41 22 749 01 11 Fax +41 22 749 09 47 [email protected] www.iso.org PrganizationforStandardization ensee-ZHEJIANG INSTOF STANDARDIzARoISQa7 - All rights reserved Not for Resale, 2017/8/21 02:13:01 etworking permitted without license from IHS IS0 21148:2017(E) Contents Page Foreword ..V Introduction. ...vi 1 Scope. 2 Normative references ..1 3 Terms and definitions 4 Premises .1 4.1 Test areas. .1 4.2 Additional areas .2 4.3 Location of the premises .2 4.4 Equipping the premises 4.5 Maintenance .3 5 Equipment. .3 5.1 General .3 5.2 Microbiological cabinets 3 5.3 Balances .3 5.4 Homogenizer 4 5.5 pH-meter. .4 5.6 Autoclave. .4 5.7 Incubator .4 5.8 Waterbaths 5.9 Refrigerator or cold-storage room. .4 5.10 Freezer ..4 5.11 Sterilizing oven .5 5.12 Colony-counting device 5.13 Other equipment. ..5 6 Strains of microorganisms 5 7 Personnel ..6 7.1 Competence ..6 7.2 Hygiene. .. 6 8 Preparation of the apparatus and glassware .6 8.1 Preparation . 6 8.2 Sterilization .6 8.2.1 Sterilization by dry heat ..6 8.2.2 Sterilization by moist heat .7 8.3 Disposable apparatus. .7 8.4 Management of clean apparatus and glassware ..7 8.5 Management of sterile apparatus and glassware. .7 8.6 Treatment of contaminated material. 8.7 Washing. .7 Preparation and sterilization of culture media and reagents. .8 9.1 General ..8 9.2 Water. .8 9.3 Preparation of culture media. 9.3.1 General. 9.3.2 Rehydration .8 9.3.3 Measurement of pH .8 9.3.4 Dispensing. .8 9.4 Sterilization .9 9.4.1 General .9 9.4.2 Sterilization by moist heat 9.4.3 Sterilization by filtration. 9 iii nsee=ZHEJIANG INST OF STANDARDIZATION C1 5956617 d without license from IHS Not for Resale, 2017/8/21 02:13:01