ISO INTERNATIONAL STANDARD 16373-2 First edition 2014-06-01 Textiles Dyestuffs - Part 2: General method for the determination of extractable dyestuffs including allergenic and carcinogenic dyestuffs (method using pyridine-water) Textiles - Colorants - Partie 2: Methode générale de détermination des colorants extractibles, notamment les colorants allergenes et cancérigenes (méthode utilisant un mélange pyridine/eau) Reference number IS0 16373-2:2014(E) ISO @ IS0 2014 I without license from IHS Not for Resale IS0 16373-2:2014(E) COPYRIGHTPROTECTEDDOCUMENT @ISO2014 All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address below or ISO's member body in the country of the requester. ISO copyright office Case postale 56 : CH-1211 Geneva 20 Tel. + 41 22 749 01 11 Fax +41 22 749 09 47 E-mail [email protected] Web www.iso.org Published in Switzerland @ IS0 2014 - All rights reserved No reproduction or networking permited without license from IHS Not for Resale IS0 16373-2:2014(E) Contents Page Foreword ..iv Introduction. ..V 1 Scope. ..1 2 Normative references ..1 3 Terms and definitions 4 Principle ..1 5 Safety precautions ..1 6 Reagents ..2 7 Apparatus .2 7.1 Apparatus and auxiliaries for sample preparation .2 7.2 Chromatographic equipment (selected from the following) .2 8 Procedure .3 8.1 Preparation of test specimen. .3 8.2 Extraction .3 8.3 Detection and quantification of dyestuff .3 8.4 Calibration ..3 9 Calculation and expression of the results ..4 10 Test report .4 Annex A (normative) List of carcinogenic dyestuffs Annex B (normative) List of allergenic and other dyestuffs .6 Annex C (normative) Calculation ..7 Annex D (informative) Examples of chromatographic methods ..8 Annex E (informative) Reliability of the method ..17 Annex F (informative) Multiple-extraction of several textile fibres with pyridine/water ..18 Bibliography .20 ii thout license from IHS Not for Resale