ISO INTERNATIONAL STANDARD 14442 Second edition 2006-04-01 Water quality Guidelines for algal growth inhibition tests with poorly soluble materials,volatile compounds, metals and waste water Qualitede I'eau-Lignes directrices pouressais d'inhibition de la croissancealgale avecdesmatierespeu solubles, des composes volatils,desmetauxetdeseauxrésiduaires Referencenumber ISO 14442:2006(E) @ISO2006 ISO14442:2006(E) PDF disclaimer This PDF file may contain embedded typefaces. In accordance with Adobe's licensing policy, this file may be printed or viewed but downloading this file, parties accept therein the responsibility of not infringing Adobe's licensing policy. The ISO Central Secretariat accepts no liability in this area. Adobe is a trademark of Adobe Systems Incorporated. Details of the software products used to create this PDF file can be found in the General Info relative to the file; the PDF-creation the unlikely event that a problem relating to it is found, please infom the Central Secretariat at the address given below. ISO2006 All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying and microfilm, without permission in writing from either ISO at the address below or IsO's member body in the country of the requester. ISO copyright office Case postale 56·CH-1211Geneva 20 Tel. + 41 22 749 01 11 Fax + 41 22 749 09 47 E-mail [email protected] Web www.iso.org Published in Switzerland ii @ ISO 2006 - All rights reserved ISO14442:2006(E) Contents Page Foreword. 1 Scope 2 Normative references 3 Analytical characterization of test materials and confirmation of concentrations and stability .... 4 Poorly soluble organic substances .... 4.1 General. 4.2 Preparation of saturated and supersaturated solutions..... 4.3 Solvent addition.... 4.4 Dispersion using an emulsifying agent.. 4.5 Interference with algal growth and its measurement.. 5 Poorly solublemixtures of organic substances 5.1 General..... 5.2 Preparation of test media.. 5.3 Test performance.. 6 Poorly soluble inorganic materials 7 Volatile substances 7.1 8 7.2 Test system and growth medium. 7.3 Testperformance. 7.4 Interference with algal growth. 9 8 Waste waters andenvironmental aqueous samples. 9 Colouredand/orturbid samples 10 10 Metalsand metal compounds. 10.1 Introduction.... 11 10.2 Modification of algal growth inhibition test procedures for testing materials containing heavy metals ... 11 11 pH buffering.. .12 12 Interpretation of the results.. 13 Bibliography. 14 @ ISO 2006 - All rights reserved ii ISO 14442:2006(E) Foreword IsO (the International Organization for Standardization) is a worldwide federation of national standards bodies InternationalElectrotechnicalCommission(lEC)onallmattersofelectrotechnicalstandardization. International Standards are drafted in accordance with the rules given in the ISO/lEC Directives, Part 2. The main task of technical committees is to prepare International Standards. Draft International Standards adopted by the technical committees are circulated to the member bodies for voting. Publication as an International Standard requires approval by at least75%ofthe memberbodies castinga vote Attention is drawn to the possibility that some of the elements of this document may be the subject of patent ISO 14442 was prepared by Technical Committee ISO/TC 147, Water quality, Subcommittee SC 5, Biological methods This second edition cancels and replaces the first edition (isO 14442:1999), which has been technically revised. iv @ ISO 2006 - All rights reserved INTERNATIONAL STANDARD ISO14442:2006(E) Water quality Guidelines for algal growth inhibition tests with poorly soluble materials, volatile compounds, metals and waste water WARNING - Persons using this International Standard should be familiar with normal laboratory practice.This International Standard does not purport to address all of the safety problems, if a