ISO INTERNATIONAL STANDARD 27368 First edition 2008-08-15 Analysis of blood for asphyxiant toxicants Carbon monoxide and hydrogen cyanide Analyse du sang pour substances toxiques asphyxiantes Monoxyde de carbone et acide cyanhydrique Reference number ISO 27368:2008(E) @ ISO 2008 by IHS under lic Not for Resale ISO27368:2008(E) PDF disclaimer This PDF file may contain embedded typefaces. In accordance with Adobe's licensing policy, this file may be printed or viewed but shall not be edited unless the typefaces which are embedded are licensed to and installed on the computer performing the editing. In accepts no liability in this area. Adobe is a trademark of Adobe Systems Incorporated. Details of the software products used to create this PDF file can be found in the General Info relative to the file; the PDF-creation parameters were optimized for printing. Every care has been taken to ensure that the file is suitable for use by IsO member bodies. In the unlikely event that a problem relating to it is found, please inform the Central Secretariat at the address given below COPYRIGHTPROTECTEDDOCUMENT @ ISO2008 All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized in any form or by any means, IsO's member body in the country of the requester. ISO copyright office Case postale 56. CH-1211 Geneva 20 Tel. + 41 22 749 01 11 Fax + 41 22 749 09 47 E-mail copyright@iso.org Web www.iso.org Published in Switzerland @ Iso 2008 - All rights reserved I without license from IHS Not for Resale ISO 27368:2008(E) Contents Page Foreword. Introduction 1 Scope 2 Normative references . 3 Terms and definitions 4 Symbols and abbreviated terms 5 5 Blood samples 5.1 General.. 5.2 Sample condition. 5.3 Sample collection .. 5.4 Sample storage . 5.5 Sample analysis .. 6 Materials . 7 Common quality analytical elements.. 7.1 General...... 7.2 Qualitative, quantitative and confirmatory analyses ...... 7.3 Replicate analyses.. 7.4 Analytical batch ... 7.5 Open controls. 7.6 Calibrators 8 Measurement of co in blood as COHb.. 8.1 COHb by whole-blood oximeters ... 8 8.2 COHb by palladium chloride reduction .. 10 8.3 coHb by visible spectrophotometry (using calibration curve) . 12 8.4 CoHb by visible spectrophotometry (with co saturation)... .14 8.5 CoHb by visible spectrophotometry (without co saturation).... .16 8.6 ionization detection ..... 8.7 CoHb by headspace gas chromatography Thermal conductivity detection... 9 Measurement of HCN in blood as CN-. .. 23 9.1 CN- by colourimetric method (p-nitrobenzaldehyde and o-dinitrobenzene)..... .23 9.2 CN- by visible spectrophotometry... 9.3 CN- as HcN by headspace gas chromatography - Nitrogen phosphorous detection... 9.4 CN- by headspace gas chromatography Electron capture detection ... -31 9.5 CN- by spectrophotofluorimetry or high-performance liquid chromatography using a fluorescence detector.... .33 9.6 CN- by high-performance liquid chromatography-mass spectrometry.. 37 Annex A (normative) Analytical report pro forma... .41 Annex B (informative) Additional aspects of analytical methods .... 43 Annex C (informative) Interpretation of results. 47 Bibliography 52 CopyrightInenationalOganzaadardizaoghtsreserved ii Not for Resale