ISO INTERNATIONAL STANDARD 20665 First edition 2008-12-15 Water quality Determination of chronic toxicity to Ceriodaphnia dubia Qualité de I'eau Determination de la toxicite chronique vis-a-vis de Ceriodaphnia dubia Reference number ISO 20665:2008(E) @ ISO 2008 yIHSunder itted without license from IHS Not for Resale ISO20665:2008(E) PDF disclaimer This PDF file may contain embedded typefaces. In accordance with Adobe's licensing policy, this file may be printed or viewed but shall not be edited unless the typefaces which are embedded are licensed to and installed on the computer performing the editing. In accepts no liability in this area. Adobe is a trademark of Adobe Systems Incorporated. Details of the software products used to create this PDF file can be found in the General Info relative to the file; the PDF-creation parameters were optimized for printing. Every care has been taken to ensure that the file is suitable for use by IsO member bodies. In COPYRIGHTPROTECTEDDOCUMENT @ ISO2008 All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying and microfilm, without permission in writing from either isO at the address below or IsO's memberbody in the country of the requester. ISO copyright office Case postale 56.CH-1211Geneva 20 Tel. + 4122 749 01 11 Fax + 41 22 749 09 47 E-mail [email protected] Web www.iso.org Published in Switzerland @ ISO 2008 - All rights reserved ted without license from IHS Not for Resale ISO 20665:2008(E) Contents Page Foreword Introduction 1 Scope 2 Normative references 3 Terms and definitions 4 Principle... 5 Test environment.. 6 Reagents, test organisms and media. 7 Apparatus 8 Sampling and samples. 9 Procedure 10 Expression of results 11 Validity criteria .. 12 Precision.. 13 Test report .... 12 Annex A (normative) Preparation of the ELENDT M4 medium. Annex B (normative) Preparation of the moderately hard water medium..... Annex C (informative) Preparation of the LC OLiGO medium... Annex D (normative) Procedure for preparing YCT 18 Annex E (informative) Data collection sheet .. Bibliography ... 21 Copyright International Organization forstandardizallon'ghts reserved ii from IHS Not for Res