ISO/TS TECHNICAL SPECIFICATION 13896 First edition 2012-09-01 Soil quality Determination of linear alkylbenzene sulfonate (LAS) - Method by HPLC with fluorescence detection (LC-FLD) and mass selective detection (LC-MSD) Qualité du sol - Determination des sulfonates d'alkyl benzene linéaires (SAL) - Méthode par chromatographie liquide a haute performance (CLHP) avec détection par fluorescence (CL-DFL) et détection sélective de la masse (CL-DSM) Reference number IS0/TS 13896:2012(E) LSO IS02012 It license from IHS Not for Resale IS0/TS 13896:2012(E) COPYRIGHTPROTECTEDDOCUMENT ISO2012 All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying and microfilm, without permission in writing from either IsO at the addressbeloworIso'smemberbodyinthecountryoftherequester. ISO copyright office Case postale 56:CH-1211 Geneva 20 Tel.+41227490111 Fax +41 22 749 09 47 E-mail [email protected] Web www.iso.org Published in Switzerland @ IS0 2012 - All rights reserved No reproduction networking permited without license from IHS Not for Resale IS0/TS 13896:2012(E) Contents Page Foreword ..iv Introduction. ..V 1 Scope. .1 2 Normative references ..1 3 Terms and definitions 4 Principle ..2 5 Interferences .2 5.1 Interferences from sampling. .2 5.2 Interferences by HPLC-FLD and HPLC-MS ..2 6 Reagents ..3 7 Apparatus ..5 8 Sample storage and sample pretreatment .6 8.1 Sample storage. .6 8.2 Sample pretreatment. .6 9 Procedure .6 9.1 Extraction . 6 9.2 Concentration (optional) .7 9.3 Clean-up (optional) .1 9.4 Blanktest .7 9.5 HPLCanalysis .7 9.6 Calibration ..8 10 Calculation and expression of results .9 10.1 General .9 10.2 Calibration 10 10.3 Calculation 10 11 Precision ..11 12 Test report ..11 Annex A (informative) Repeatability and reproducibility data ..12 Annex B (informative) Examples of chromatographic conditions and chromatograms ..14 Annex C (informative) Examples of clean-up procedures ..17 Bibliography. ..18 Sta iii se with IS thout license from IHS Not for Resale